School of Life Sciences, The Engineering Research Center of Sustainable, Development and Utilization of Biomass Energy, Yunnan Normal University, Kunming 650500, China.
Molecules. 2018 Mar 29;23(4):788. doi: 10.3390/molecules23040788.
The tea catechin epigallocatechin-3-gallate (EGCG) proved to be the most potent physiologically active tea compound in vitro. It possesses antioxidant as well as pro-oxidant properties. EGCG has the effect of inducing apoptosis of tumor cells and inhibiting cell proliferation. Whether this effect is associated with the antioxidant or pro-oxidative effects of EGCG affecting the genome stability of normal and cancer cells has not been confirmed. Here, we selected Human normal colon epithelial cells NCM460 and colon adenocarcinoma cells COLO205 to investigate the effects of EGCG (0−40 μg/mL) on the genome stability and cell growth status. Chromosomal instability (CIN), nuclear division index (NDI), and apoptosis was measured by cytokinesis-block micronucleus assay (CBMN), and the expression of core genes in mismatch repair ( and ) was examined by RT-qPCR. We found that EGCG significantly reduced CIN and apoptosis rate of NCM460 at all concentrations (5−40 μg/mL) and treatment time, EGCG at 5 μg/mL promoted cell division; EGCG could significantly induce chromosome instability in COLO205 cells and trigger apoptosis and inhibition of cell division. These results suggest that EGCG exhibits different genetic and cytological effects in normal and colon cancer cells.
儿茶素表没食子儿茶素没食子酸酯（EGCG）被证明是体外最具生理活性的茶类化合物。它具有抗氧化和促氧化特性。EGCG 具有诱导肿瘤细胞凋亡和抑制细胞增殖的作用。这种作用是否与 EGCG 的抗氧化或促氧化作用影响正常和癌细胞的基因组稳定性尚未得到证实。在这里，我们选择人正常结肠上皮细胞 NCM460 和结肠腺癌细胞 COLO205 来研究 EGCG（0-40μg/ml）对基因组稳定性和细胞生长状态的影响。通过胞质分裂阻断微核试验（CBMN）测量染色体不稳定（CIN）、核分裂指数（NDI）和细胞凋亡，并用 RT-qPCR 检测错配修复核心基因（和）的表达。我们发现 EGCG 显著降低了 NCM460 在所有浓度（5-40μg/ml）和处理时间的 CIN 和细胞凋亡率，EGCG 在 5μg/ml 时促进细胞分裂；EGCG 可显著诱导 COLO205 细胞染色体不稳定，并引发细胞凋亡和抑制细胞分裂。这些结果表明，EGCG 在正常和结肠癌细胞中表现出不同的遗传和细胞遗传学效应。
