Yunnan Institute of Food Safety, Kunming University of Science and Technology, No. 727 South Jingming Road, Kunming 650500, Yunnan, China.
Nutrients. 2018 Mar 28;10(4):420. doi: 10.3390/nu10040420.
A previous study has shown that tilapia fish skin gelatin hydrolysates inhibited photoaging in vivo, and that, Leu-Ser-Gly-Tyr-Gly-Pro (LSGYGP) identified in the hydrolysate had a high hydroxyl radical scavenging activity. In this study, activities of LSGYGP were further evaluated using ultraviolet B (UVB)-induced mouse embryonic fibroblasts (MEFs). UVB irradiation significantly increased the intercellular reactive oxygen species (ROS) production and matrix metalloproteinases (MMPs) activities and decreased the content of collagen in MEFs. LSGYGP reduced the intercellular ROS generation in UVB-induced MEFs. Meanwhile, the decrease of superoxide dismutase (SOD) activity and the increase of malondiaidehyde (MDA) content were inhibited by LSGYGP. LSGYGP reduced MMP-1 and MMP-9 activities in a dose-dependent manner. Molecular docking simulation indicated that LSGYGP inhibited MMPs activities by docking the active sites of MMP-1 and MMP-9. Furthermore, LSGYGP also affected the intercellular phosphorylation of UVB-induced the mitogen-activated protein kinase pathway. LSGYGP could protect collagen synthesis in MEFs under UVB irradiation by inhibiting oxidative stress and regulating MMPs activities.
先前的研究表明，罗非鱼皮明胶水解物可抑制体内光老化，并且在水解物中鉴定出的亮氨酸-丝氨酸-甘氨酸-酪氨酸-甘氨酸-脯氨酸（LSGYGP）具有很高的羟基自由基清除活性。在这项研究中，使用紫外线 B（UVB）诱导的小鼠胚胎成纤维细胞（MEFs）进一步评估了 LSGYGP 的活性。UVB 照射显著增加了细胞间活性氧（ROS）的产生和基质金属蛋白酶（MMPs）的活性，同时降低了 MEFs 中胶原蛋白的含量。LSGYGP 减少了 UVB 诱导的 MEFs 中的细胞间 ROS 生成。同时，LSGYGP 抑制了超氧化物歧化酶（SOD）活性的降低和丙二醛（MDA）含量的增加。LSGYGP 以剂量依赖的方式降低了 MMP-1 和 MMP-9 的活性。分子对接模拟表明，LSGYGP 通过对接 MMP-1 和 MMP-9 的活性部位来抑制 MMPs 的活性。此外，LSGYGP 还影响了 UVB 诱导的丝裂原活化蛋白激酶途径的细胞间磷酸化。LSGYGP 可通过抑制氧化应激和调节 MMPs 活性来保护 MEFs 在 UVB 照射下的胶原蛋白合成。
