Department of Genetic Engineering , Institute of Cell Biology and Genetic Engineering of National Academy of Sciences of Ukraine (NASU) , Academika Zabolotnoho, 148 , 03143 Kyiv , Ukraine.
Department of Analytical Chemistry, Institute C-1, Faculty of Chemical Engineering and Technology , Cracow University of Technology , ul. Warszawska 24 , Cracow 31-155 , Poland.
J Agric Food Chem. 2018 Apr 18;66(15):3870-3879. doi: 10.1021/acs.jafc.8b01014. Epub 2018 Apr 9.
Betacyanins and betaxanthins were characterized and determined in an intensely pigmented red-colored callus culture of Celosia cristata L. (Amaranthaceae). A new malonyl derivative, 6'- O-malonyl-amaranthin (celoscristatin) was isolated and identified by spectroscopic and mass spectrometric techniques. Its stereoisomer, 4'- O-malonyl-amaranthin (celoscristatin acyl-migrated), as well as its 15 R diastereomer were also detected in the callus as a result of the malonyl group migration in celoscristatin/isoceloscristatin, respectively. Amaranthin occurs in the callus as the major betacyanin, followed by celoscristatin, betanin, phyllocactin, and other minor betacyanins. The effect of different carbon sources on the growth rates of the Celosia callus as well as on betalains profiles in the callus cultures was studied. High dopamine content in the callus culture was determined and compared with the content in C. cristata inflorescences. The dopamine-based betalain (miraxanthin V) was detected as the main betaxanthin in the callus, however, at a concentration level much lower than that of the identified betacyanins. The studied callus culture of C. cristata can accumulate betalains in amounts which approach the quantities produced by most known high-yielding plant species.
研究了不同碳源对鸡冠花愈伤组织生长速度和培养物中甜菜碱谱的影响。测定了愈伤组织中的高多巴胺含量，并与鸡冠花花序中的含量进行了比较。在愈伤组织中检测到以多巴胺为基础的甜菜碱（米若卡林 V）作为主要的甜菜黄素，但浓度远低于所鉴定的甜菜碱。所研究的鸡冠花愈伤组织可以积累甜菜碱，其数量接近大多数已知高产植物物种产生的数量。
