College of Plant Protection , Shenyang Agricultural University , Shenyang 110866 , People's Republic of China.
Engineering & Technological Research Center of Biopesticide for Liaoning Province, Shenyang 110866 , People's Republic of China.
J Agric Food Chem. 2018 Apr 18;66(15):3833-3839. doi: 10.1021/acs.jafc.8b00253. Epub 2018 Apr 5.
For decades, plant parasitic nematodes have caused serious damage to crop production. Most nematicides are banned because of their negative impacts on the environment and public health. The repeated application of the few commercially available nematicides has caused more incidences of nematicide resistance. To seek novel nematicides, seven linear peptides named rhabdopeptides I-O, 1-7, were isolated from culture broth of Xenorhabdus budapestensis SN84. The structures of the peptides were elucidated on the basis of extensive mass spectrometry (MS), and nuclear magnetic resonance analyses. 3, 4, and 7 were novel compounds. 1, 2, 5, and 6 were isolated and purified for the first time, despite being previously elucidated from an extract mixture based on labeling and MS experiments. All seven compounds were tested for their nematicidal activities against the second-stage juveniles (J2) of Meloidogyne incognita using 24-microwell plates. Rhabdopeptide J, 2, demonstrated strong inhibitory activity with an LC value of 27.8 μg/mL. Rhabdopeptide K, 3, and M, 5, showed moderate inhibitory activity with LC values of 46.3 and 42.4 μg/mL, respectively.
几十年来，植物寄生线虫一直对作物生产造成严重破坏。由于对环境和公众健康的负面影响，大多数杀线虫剂已被禁止使用。少数市售杀线虫剂的反复使用导致了更多的杀线虫剂抗性事件。为了寻找新型杀线虫剂，从 Xenorhabdus budapestensis SN84 的培养液中分离出了七种线性肽，命名为 rhabdopeptides I-O、1-7。基于广泛的质谱（MS）和核磁共振分析，确定了这些肽的结构。3、4 和 7 是新化合物。1、2、5 和 6 尽管以前是根据标记和 MS 实验从提取物混合物中分离和纯化出来的，但这次也是首次被分离和纯化出来。所有七种化合物都在 24 孔板上用南方根结线虫（Meloidogyne incognita）的第二龄幼虫（J2）测试了它们的杀线虫活性。rhabdopeptide J，2，表现出强烈的抑制活性，LC 值为 27.8 μg/mL。rhabdopeptide K，3 和 M，5，表现出中等抑制活性，LC 值分别为 46.3 和 42.4 μg/mL。
