Wang Yinan, Yang Di, Yu Zhiguo
College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China.
Engineering & Technological Research Center of Biopesticide for Liaoning Province, Shenyang 110866, China.
Microorganisms. 2023 Feb 28;11(3):616. doi: 10.3390/microorganisms11030616.
causes southern corn leaf blight and inflicts huge losses on maize production. In order to search for new natural products from insect gut bacteria to control plant fungal disease, 86 actinomycetes were isolated from more than 50 insect guts, in which crude extract of strain SN5431 showed significant inhibition of the mycelial growth of . The strain was identified and named as sp. SN5431. Six compounds were obtained from the crude extract of strain SN5431, which includes five new -butyrolactones named as tiuslactone A-E (-), and one new long chain ester named as tiusester (). Their structures were determined using NMR and HRESIMS data and then combined with the spectroscopic data of known similar compounds. Tiuslactone B () showed powerful antifungal activity against . These results indicated metabolites of insect gut bacteria have the potential to be the leading compounds for the control of corn leaf blight.
引起玉米叶斑病并给玉米生产造成巨大损失。为了从昆虫肠道细菌中寻找控制植物真菌病害的新天然产物，从50多种昆虫肠道中分离出86株放线菌，其中菌株SN5431的粗提物对[病原菌名称缺失]的菌丝生长有显著抑制作用。该菌株被鉴定并命名为[属名缺失] sp. SN5431。从菌株SN5431的粗提物中获得了6种化合物，其中包括5种新的γ-丁内酯，命名为tiuslactone A - E (-)，以及一种新的长链酯，命名为tiusester ()。利用核磁共振（NMR）和高分辨电喷雾电离质谱（HRESIMS）数据确定了它们的结构，然后结合已知类似化合物的光谱数据。Tiuslactone B () 对[病原菌名称缺失]显示出强大的抗真菌活性。这些结果表明昆虫肠道细菌的代谢产物有潜力成为控制玉米叶斑病的先导化合物。
