Glazebrook J A, Mitchell K, Radford A
Department of Genetics, University of Leeds, UK.
Mol Gen Genet. 1987 Sep;209(2):399-402. doi: 10.1007/BF00329672.
By means of S1 nuclease mapping, one transcription origin and three termini are identified for the pyr-4 gene of Neurospora crassa, the same origin being used also by Escherichia coli on the cloned gene. Translation of the clone in mini-cells gives a 50,000 dalton gene product, the same size as that determined for the Neurospora native enzyme. Putative CAAT and TATA boxes, and upstream and downstream potential secondary structures, are identified.
通过S1核酸酶图谱分析，确定了粗糙脉孢菌pyr-4基因的一个转录起始点和三个末端，大肠杆菌在克隆基因上也使用相同的起始点。在小细胞中对该克隆进行翻译，得到一个50,000道尔顿的基因产物，其大小与粗糙脉孢菌天然酶的大小相同。确定了推定的CAAT盒和TATA盒，以及上游和下游潜在的二级结构。
