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优化中空纤维生物反应器中的T细胞扩增

Optimizing T Cell Expansion in a Hollow-Fiber Bioreactor.

作者信息

Nankervis Brian, Jones Mark, Vang Boah, Brent Rice R, Coeshott Claire, Beltzer Jim

机构信息

Terumo BCT, Inc., 11158 West Collins Avenue, Lakewood, CO 80215 USA.

出版信息

Curr Stem Cell Rep. 2018;4(1):46-51. doi: 10.1007/s40778-018-0116-x. Epub 2018 Feb 27.

DOI:10.1007/s40778-018-0116-x
PMID:29600161
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5866265/
Abstract

PURPOSE OF REVIEW

Recent developments in regenerative medicine have precipitated the need to expand gene-modified human T cells to numbers that exceed the capacity of well-plate-based, and flask-based processes. This review discusses the changes in process development that are needed to meet the cell expansion requirements by utilizing . Maintenance of cell proliferation over long periods can become limited by unfilled demands for nutrients and oxygen and by the accumulation of waste products in the local environment.

RECENT FINDINGS

Perfusion feeding, improved gas exchange, and the efficient removal of lactate can increase the yield of T cells from an average of 10.8E +09 to more than 28E +09 in only 10 days.

SUMMARY

Aggressively feeding cells and actively keeping cells in the bioreactor improves gas exchange and metabolite management over semi-static methods. The ability to remove the environmental constraints that can limit cell expansion by using a two-chamber hollow-fiber bioreactor will be discussed.

摘要

综述目的

再生医学的最新进展促使人们需要将基因编辑的人类T细胞扩增到超过基于微孔板和培养瓶的培养方法所能达到的数量。本综述讨论了通过利用……来满足细胞扩增需求所需的工艺开发变化。长期维持细胞增殖可能会受到营养物质和氧气需求未得到满足以及局部环境中废物积累的限制。

最新发现

灌注喂养、改善气体交换以及有效去除乳酸，可使T细胞产量在短短10天内从平均10.8E +09增加到超过28E +09。

总结

与半静态方法相比，积极喂养细胞并主动将细胞保留在生物反应器中可改善气体交换和代谢物管理。将讨论使用双腔中空纤维生物反应器消除可能限制细胞扩增的环境限制的能力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cef4/5866265/d239ee35eb96/40778_2018_116_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cef4/5866265/74b42b83a40f/40778_2018_116_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cef4/5866265/d239ee35eb96/40778_2018_116_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cef4/5866265/74b42b83a40f/40778_2018_116_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cef4/5866265/d239ee35eb96/40778_2018_116_Fig2_HTML.jpg

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