Denlinger Rachel L, Smith Tracy T, Murphy Sharon E, Koopmeiners Joseph S, Benowitz Neal L, Hatsukami Dorothy K, Pacek Lauren R, Colino Cirielle, Cwalina Samantha N, Donny Eric C
Project Manager, Department of Psychology, University of Pittsburgh, Pittsburgh, PA.
Graduate Student, Department of Psychology, University of Pittsburgh, Pittsburgh, PA.
Tob Regul Sci. 2016 Apr;2(2):186-203. doi: 10.18001/TRS.2.2.9.
Research using very low nicotine content (VLNC) cigarettes has shown that participants underreport use of non-study cigarettes. Biomarkers of nicotine exposure could be used to verify compliance with VLNC cigarettes. This study aimed to characterize biomarkers of exposure when participants exclusively use VLNC cigarettes.
23 participants stayed in a hotel that permitted smoking for 5 days and 4 nights. They were provided 2 packs of VLNC cigarettes each day (0.4 mg of nicotine/g of tobacco; Spectrum cigarettes) and did not have access to other tobacco products. 24-hour urine samples were collected to assess exposure to nicotine and anatabine.
After 4 days of exclusive use, the geometric means for urinary total cotinine, total nicotine equivalents (TNE), and anatabine were 1.13 nmol/ml (92% reduction), 3.17 nmol/ml (94% reduction) and 0.0031 nmol/ml (93% reduction). The population estimates of the 95 percentile of cotinine, TNE, and anatabine levels were 2.69, 6.41, and 0.0099 nmol/ml, respectively.
Study participants exclusively smoking 0.4 mg/g Spectrum cigarettes are unlikely to have biomarker values above these levels. The data presented here will be valuable to researchers conducting research on use of VLNC cigarettes.
使用极低尼古丁含量（VLNC）香烟的研究表明，参与者会少报非研究用香烟的使用情况。尼古丁暴露生物标志物可用于验证对VLNC香烟的依从性。本研究旨在描述参与者仅使用VLNC香烟时的暴露生物标志物特征。
23名参与者在一家允许吸烟的酒店住宿5天4夜。每天为他们提供2包VLNC香烟（每克烟草含0.4毫克尼古丁；Spectrum香烟），且他们无法获得其他烟草制品。收集24小时尿液样本以评估尼古丁和去甲替林的暴露情况。
仅使用4天后，尿中总可替宁、总尼古丁当量（TNE）和去甲替林的几何平均值分别为1.13纳摩尔/毫升（降低92%）、3.17纳摩尔/毫升（降低94%）和0.0031纳摩尔/毫升（降低93%）。可替宁、TNE和去甲替林水平第95百分位数的总体估计值分别为2.69、6.41和0.0099纳摩尔/毫升。
仅吸食每克含0.4毫克Spectrum香烟的研究参与者，其生物标志物值不太可能高于这些水平。此处呈现的数据对于开展VLNC香烟使用研究的人员将很有价值。
