对“体内CRISPR-Cas9编辑后出现意外突变”的回应 - Suppr

Response to "Unexpected mutations after CRISPR-Cas9 editing in vivo".

作者信息

Wilson Christopher J, Fennell Tim, Bothmer Anne, Maeder Morgan L, Reyon Deepak, Cotta-Ramusino Cecilia, Fernandez Cecilia A, Marco Eugenio, Barrera Luis A, Jayaram Hariharan, Albright Charles F, Cox Gerald F, Church George M, Myer Vic E

机构信息

Editas Medicine, Cambridge, Massachusetts, USA.

Fulcrum Genomics, Somerville, Massachusetts, USA.

出版信息

Nat Methods. 2018 Apr;15(4):236-237. doi: 10.1038/nmeth.4552. Epub 2018 Mar 30.

DOI:10.1038/nmeth.4552

PMID:29600989

Abstract

摘要

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本文引用的文献

Unexpected mutations after CRISPR-Cas9 editing in vivo.体内CRISPR-Cas9编辑后出现的意外突变。

Nat Methods. 2017 May 30;14(6):547-548. doi: 10.1038/nmeth.4293.

Genetic and epigenetic variation among inbred mouse littermates: identification of inter-individual differentially methylated regions.近交系小鼠同窝仔之间的遗传和表观遗传变异：个体间差异甲基化区域的鉴定。

Epigenetics Chromatin. 2015 Dec 12;8:54. doi: 10.1186/s13072-015-0047-z. eCollection 2015.

Off-target mutations are rare in Cas9-modified mice.脱靶突变在经Cas9修饰的小鼠中很少见。

Nat Methods. 2015 Jun;12(6):479. doi: 10.1038/nmeth.3408.

Low incidence of off-target mutations in individual CRISPR-Cas9 and TALEN targeted human stem cell clones detected by whole-genome sequencing.通过全基因组测序检测到，单个CRISPR-Cas9和TALEN靶向的人类干细胞克隆中脱靶突变的发生率较低。

Cell Stem Cell. 2014 Jul 3;15(1):27-30. doi: 10.1016/j.stem.2014.04.020.

Whole-genome sequencing analysis reveals high specificity of CRISPR/Cas9 and TALEN-based genome editing in human iPSCs.全基因组测序分析揭示了CRISPR/Cas9和基于TALEN的基因组编辑在人诱导多能干细胞中的高特异性。

Cell Stem Cell. 2014 Jul 3;15(1):12-3. doi: 10.1016/j.stem.2014.06.011.

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Response to "Unexpected mutations after CRISPR-Cas9 editing in vivo".

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