Opt Lett. 2018 Apr 1;43(7):1423-1426. doi: 10.1364/OL.43.001423.
Measuring three-dimensional nanoscale cellular structures is challenging, especially when the structure is dynamic. Owing to the informative total internal reflection fluorescence (TIRF) imaging under varied illumination angles, multi-angle (MA) TIRF has been examined to offer a nanoscale axial and a subsecond temporal resolution. However, conventional MA-TIRF still performs badly in lateral resolution and fails to characterize the depth image in densely distributed regions. Here, we emphasize the lateral super-resolution in the MA-TIRF, exampled by simply introducing polarization modulation into the illumination procedure. Equipped with a sparsity and accelerated proximal algorithm, we examine a more precise 3D sample structure compared with previous methods, enabling live cell imaging with a temporal resolution of 2 s and recovering high-resolution mitochondria fission and fusion processes. We also shared the recovery program, which is the first open-source recovery code for MA-TIRF, to the best of our knowledge.
测量三维纳米级细胞结构具有挑战性，特别是当结构具有动态性时。由于在不同照明角度下具有信息丰富的全内反射荧光（TIRF）成像，多角度（MA）TIRF 已被检查以提供纳米级轴向和亚秒级时间分辨率。然而，传统的 MA-TIRF 在横向分辨率方面表现仍不佳，无法对密集分布区域的深度图像进行特征描述。在这里，我们通过简单地将偏振调制引入照明过程来强调 MA-TIRF 中的横向超分辨率。配备稀疏和加速近端算法，我们检查了比以前的方法更精确的 3D 样品结构，使具有 2 秒时间分辨率的活细胞成像成为可能，并恢复了高分辨率的线粒体裂变和融合过程。我们还共享了恢复程序，据我们所知，这是 MA-TIRF 的第一个开源恢复代码。
