多色活细胞超分辨率体积成像与多角度干涉显微镜。

State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou, Zhejiang, 310027, China.

Shanxi Provincial Key Laboratory for Biomedical Imaging and Big Data, North University of China, Taiyuan, Shanxi, 030051, China.

Nat Commun. 2018 Nov 16;9(1):4818. doi: 10.1038/s41467-018-07244-4.

Imaging and tracking of near-surface three-dimensional volumetric nanoscale dynamic processes of live cells remains a challenging problem. In this paper, we propose a multi-color live-cell near-surface-volume super-resolution microscopy method that combines total internal reflection fluorescence structured illumination microscopy with multi-angle evanescent light illumination. We demonstrate that our approach of multi-angle interference microscopy is perfectly adapted to studying subcellular dynamics of mitochondria and microtubule architectures during cell migration.

对活细胞近表面三维体积纳观动态过程的成像和跟踪仍然是一个具有挑战性的问题。在本文中，我们提出了一种多色活细胞近表面体积超分辨显微镜方法，该方法将全内反射荧光结构光照相术与多角度消逝光照明相结合。我们证明了我们的多角度干涉显微镜方法非常适合研究细胞迁移过程中线粒体和微管结构的亚细胞动力学。