Key Lab of Analysis and Detection for Food Safety of Ministry of Education, Fujian Provincial Key Lab of Analysis and Detection for Food Safety, College of Chemistry , Fuzhou University , Fuzhou , Fujian 350116 , China.
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection , Fujian Agriculture and Forestry University , Fuzhou 350002 , P.R. China.
Anal Chem. 2018 Apr 17;90(8):5489-5495. doi: 10.1021/acs.analchem.8b01100. Epub 2018 Apr 4.
Methyl-mercury (CHHg) and ethyl-mercury (CHHg) have much higher toxicity than Hg and can be more easily accumulated by organisms to form severe bioamplification. Hence, the specific and on-site detection of CHHg and CHHg in seafood is of great significance and a hard challenge. We herein designed two T-rich aptamers (H and H) for specifically recognizing CHHg and the total of CHHg and CHHg, respectively. In the presence of all Au, Ag, and T-rich aptamer, CHHg and CHHg specifically and preferentially bind with aptamer and thus induced the formation of alloy Ag-Au nanoparticles after reduction, which led to the color change in solution. This provided a sensing platform for the instrument-free visual discrimination and detection of CHHg and CHHg. By using H as probe, the method can be used to detect as low as 5.0 μM (equivalent to 1.0 μg Hg/g) of CHHg by bare eye observation and 0.5 μM (equivalent to 100 ng Hg/g) of CHHg by UV-visible spectrometry. By using H as probe, the method can be used to detect the total concentration of CHHg and CHHg with a visual detection limit of 5.0 μM (equivalent to 1.0 μg Hg/g) and a UV-visible spectrometry detection limit of 0.6 μM (equivalent to 120 ng Hg/g). The proposed method has been successfully used to detect CHHg and CHHg in fish muscle samples with a recovery of 101-109% and a RSD ( n = 6) < 8%. The success of this study provided a potential method for the specific and on-site detection of CHHg and CHHg in seafood by only bare eye observation.
甲基汞（CHHg）和乙基汞（CHHg）的毒性比汞高得多，更容易被生物体积累，形成严重的生物放大作用。因此，对海鲜中 CHHg 和 CHHg 的特异性和现场检测具有重要意义，也是一项艰巨的挑战。我们在此设计了两个富含 T 的适体（H 和 H），分别用于特异性识别 CHHg 和总 CHHg 和 CHHg。在所有 Au、Ag 和富含 T 的适体存在的情况下，CHHg 和 CHHg 特异性地且优先地与适体结合，从而在还原后诱导形成合金 Ag-Au 纳米粒子，导致溶液颜色发生变化。这为无仪器的视觉识别和检测 CHHg 和 CHHg 提供了一个传感平台。通过使用 H 作为探针，该方法可以通过肉眼观察检测低至 5.0 μM（相当于 1.0 μg Hg/g）的 CHHg，通过紫外可见光谱检测低至 0.5 μM（相当于 100 ng Hg/g）的 CHHg。通过使用 H 作为探针，该方法可以用于检测总浓度的 CHHg 和 CHHg，肉眼检测限为 5.0 μM（相当于 1.0 μg Hg/g），紫外可见光谱检测限为 0.6 μM（相当于 120 ng Hg/g）。该方法已成功用于检测鱼肉样品中的 CHHg 和 CHHg，回收率为 101-109%，RSD（n=6）<8%。这项研究的成功为仅通过肉眼观察对海鲜中 CHHg 和 CHHg 的特异性和现场检测提供了一种潜在的方法。
