Biomacromolecules Research Team , RIKEN Center for Sustainable Resource Science , 2-1 Hirosawa , Wako-shi , Saitama 351-0198 , Japan.
Center for Bioscience Research and Education , Utsunomiya University , 350 mine-machi , Utsunomiya , Tochigi 321-8505 , Japan.
Biomacromolecules. 2018 May 14;19(5):1582-1591. doi: 10.1021/acs.biomac.8b00323. Epub 2018 Apr 5.
Selective gene delivery into organellar genomes (mitochondrial and plastid genomes) has been limited because of a lack of appropriate platform technology, even though these organelles are essential for metabolite and energy production. Techniques for selective organellar modification are needed to functionally improve organelles and produce transplastomic/transmitochondrial plants. However, no method for mitochondrial genome modification has yet been established for multicellular organisms including plants. Likewise, modification of plastid genomes has been limited to a few plant species and algae. In the present study, we developed ionic complexes of fusion peptides containing organellar targeting signal and plasmid DNA for selective delivery of exogenous DNA into the plastid and mitochondrial genomes of intact plants. This is the first report of exogenous DNA being integrated into the mitochondrial genomes of not only plants, but also multicellular organisms in general. This fusion peptide-mediated gene delivery system is a breakthrough platform for both plant organellar biotechnology and gene therapy for mitochondrial diseases in animals.
将外源基因选择性递送到细胞器基因组（线粒体基因组和质体基因组）一直受到限制，因为缺乏合适的平台技术，尽管这些细胞器对于代谢物和能量的产生至关重要。需要选择性细胞器修饰技术来功能性地改善细胞器，并生产转质体/转线粒体植物。然而，包括植物在内的多细胞生物的线粒体基因组修饰方法尚未建立。同样，质体基因组的修饰仅限于少数几种植物和藻类。在本研究中，我们开发了融合肽的离子复合物，融合肽包含细胞器靶向信号和质粒 DNA，用于将外源 DNA 选择性递送到完整植物的质体和线粒体基因组中。这是外源 DNA 不仅整合到植物线粒体基因组中，而且整合到一般多细胞生物线粒体基因组中的首次报道。这种融合肽介导的基因传递系统是植物细胞器生物技术和动物线粒体疾病基因治疗的突破性平台。
