Department of gastroenterology, Huai'an first people's hospital, Nanjing medical university, Huai'an, China.
Department of internal medicine, Huai'an first people's hospital, Nanjing medical university, Beijing road West 6, 223300 Huai'an, Jiangsu, China.
Clin Res Hepatol Gastroenterol. 2018 Oct;42(5):478-482. doi: 10.1016/j.clinre.2018.01.006. Epub 2018 Mar 28.
Previous studies showed that down-regulation of GAS5 was involved in the development of gastric cancer (GC). However, the regulatory mechanism of down-expressed GAS5 in GC remains obscure. We aimed to investigate the role of rs145204276 of GAS5 in the development and metastasis process of GC.
853 GC patients and 954 healthy controls were recruited. The variant rs145204276 was genotyped and the Chi test was used to compare the frequency of the genotype and the allele between the patients and the controls. Odds ratio (OR) and 95% confidence intervals (95% CIs) were calculated to estimate the association of rs145204276 with the risk of development and metastasis of GC.
Patients were found to have significantly lower rate of genotype del/del than the controls (7.2% vs. 8.9%, P=0.016). The allele del was significantly associated with a decreased risk of GC (26.4% vs. 30.7%, P=0.005) with an OR of 0.81 (95% CI=0.70-0.94). Patients with allele del were less likely to develop lymph node metastasis (P=0.01), with an OR of 0.75 (95% CI=0.60-0.93). Comparably, rs145204276 was also significantly associated with a decreased risk of distant metastasis of GC (P=0.007; OR=0.55).
We confirmed that rs145204276 of GAS5 is a functional variant associated with the susceptibility and metastasis of GC. It plays a protective role in the development of GC possibly through the regulation of GAS5.
先前的研究表明，GAS5 的下调参与了胃癌（GC）的发生发展。然而，GAS5 低表达在 GC 中的调控机制仍不清楚。我们旨在研究 GAS5 的 rs145204276 变体在 GC 发展和转移过程中的作用。
共招募了 853 名 GC 患者和 954 名健康对照者。对该变体 rs145204276 进行基因分型，并用卡方检验比较患者和对照组基因型和等位基因的频率。用比值比（OR）和 95%置信区间（95%CI）来评估 rs145204276 与 GC 发展和转移风险的关联。
与对照组相比，患者的基因型 del/del 率明显较低（7.2% vs. 8.9%，P=0.016）。等位基因 del 与 GC 风险降低显著相关（26.4% vs. 30.7%，P=0.005），OR 为 0.81（95%CI=0.70-0.94）。携带等位基因 del 的患者发生淋巴结转移的可能性较低（P=0.01），OR 为 0.75（95%CI=0.60-0.93）。类似地，rs145204276 也与 GC 远处转移的风险降低显著相关（P=0.007；OR=0.55）。
我们证实 GAS5 的 rs145204276 是与 GC 易感性和转移相关的功能性变体。它可能通过调节 GAS5 在 GC 的发生发展中发挥保护作用。
