Xiang Xiaoyao, Chen Linfa, He Jiawen, Ma Guoda, Li You
Department of Urology, The Second Affiliated Hospital of Guangdong Medical University, Zhanjiang, 524001, People's Republic of China.
Department of NeUrology, Huizhou Third People's Hospital, Guangzhou Medical University, Huizhou, 516000, People's Republic of China.
J Inflamm Res. 2022 Feb 18;15:1147-1158. doi: 10.2147/JIR.S348628. eCollection 2022.
Recent studies have demonstrated that the long non-coding RNA (lncRNA) GAS5 is closely associated with the onset and progression of several tumor types, including renal cell carcinoma (RCC). This study sought to evaluate the relationship between two functional GAS5 polymorphisms (rs145204276 and rs55829688) and the risk for RCC in the Han Chinese population.
The rs145204276 and rs55829688 polymorphisms in the GAS5 promoter region were genotyped in 624 RCC patients and 655 age/sex-matched healthy participants. The association between these polymorphisms and RCC risk was then evaluated using odds ratios (ORs) and corresponding 95% confidence intervals (CIs). Additionally, quantitative RT-PCR was used to determine whether these polymorphisms were associated with changes in the levels of expression of GAS5 in 58 RCC patients.
There were significant differences in the GAS5 rs145204276 polymorphism genotype and allele frequencies between the RCC patients and controls (adjusted OR = 0.73, 95% CI = 0.61- 0.87, P = 1.8×10). When the study participants were stratified based on age, sex, BMI index, and smoking and drinking history, we found that the rs145204276 del allele was associated with a reduced risk for RCC in nondrinkers (P = 3.3×10), nonsmokers (P = 3.3×10), females (P = 3.8×10), and those who were less than 60 years old (P = 3.3×10). There was also a significant association between the rs145204276 del allele and elevated expression of GAS5 in RCC patients (P = 0.030).
The results of this study revealed an association between the rs145204276 polymorphism in the GAS5 lncRNA and the risk for the development of RCC, thus representing a potentially viable biomarker for identifying individuals at risk of developing this form of cancer.
近期研究表明，长链非编码RNA（lncRNA）GAS5与包括肾细胞癌（RCC）在内的多种肿瘤类型的发生和发展密切相关。本研究旨在评估两种功能性GAS5多态性（rs145204276和rs55829688）与中国汉族人群RCC风险之间的关系。
对624例RCC患者和655例年龄/性别匹配的健康参与者进行GAS5启动子区域rs145204276和rs55829688多态性基因分型。然后使用优势比（OR）和相应的95%置信区间（CI）评估这些多态性与RCC风险之间的关联。此外，使用定量RT-PCR确定这些多态性是否与58例RCC患者中GAS5表达水平的变化相关。
RCC患者与对照组之间GAS5 rs145204276多态性基因型和等位基因频率存在显著差异（调整后的OR = 0.73，95%CI = 0.61 - 0.87，P = 1.8×10）。当根据年龄、性别、BMI指数以及吸烟和饮酒史对研究参与者进行分层时，我们发现rs145204276缺失等位基因与不饮酒者（P = 3.3×10）、不吸烟者（P = 3.3×10）、女性（P = 3.8×10）以及年龄小于60岁者（P = 3.3×10）的RCC风险降低相关。rs145204276缺失等位基因与RCC患者中GAS5表达升高之间也存在显著关联（P = 0.030）。
本研究结果揭示了GAS5 lncRNA中rs145204276多态性与RCC发生风险之间的关联，因此代表了一种潜在可行的生物标志物，用于识别有患这种癌症风险的个体。
