GAS5基因rs145204276、NEAT1基因rs512715和MEG3基因rs4081134多态性与甲状腺乳头状癌之间的关联。
An Association Between GAS5 rs145204276, NEAT1 rs512715, and MEG3 rs4081134 Gene Polymorphisms and Papillary Thyroid Carcinoma.
作者信息
Abdi Pastaki Marjan, Salimi Saeedeh, Heidari Zahra, Saravani Mohsen
机构信息
Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
Department of Clinical Biochemistry, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
出版信息
Rep Biochem Mol Biol. 2023 Oct;12(3):487-494. doi: 10.61186/rbmb.12.3.487.
BACKGROUND
This study explores the association between growth arrest-specific 5 (GAS5) rs145204276, nuclear paraspeckle assembly transcript 1 (NEAT1) rs512715, and Maternally Expressed 3 (MEG3) rs4081134 polymorphisms and their impact on susceptibility to papillary thyroid carcinoma (PTC), considering differential expression of long noncoding RNAs (lncRNAs) in PTC.
METHODS
A case-control study involving 125 papillary thyroid carcinoma (PTC) patients and 125 controls was conducted. Genotyping of polymorphisms was performed using tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) and PCR-restriction fragment length polymorphism (PCR-RFLP) methods.
RESULTS
No significant association was found between the two groups regarding genotypes and allelic frequencies of GAS-5 145204276 and MEG3 rs4081134 polymorphisms. Genetic models also showed the same results. Regarding NEAT1 rs512715, The PTC group had more GC genotypes and over-dominant models of NEAT1 rs512715 than controls, while controls showed a higher frequency of recessive models.
CONCLUSION
GAS5 rs145204276 and MEG3 rs4081134 polymorphisms showed no significant association with papillary thyroid carcinoma (PTC) risk. In contrast, NEAT1 rs512715 exhibited a significant impact on PTC development.
背景
本研究探讨生长停滞特异性5（GAS5）rs145204276、核旁斑装配转录本1（NEAT1）rs512715和母系表达基因3（MEG3）rs4081134多态性之间的关联及其对甲状腺乳头状癌（PTC）易感性的影响，同时考虑长链非编码RNA（lncRNA）在PTC中的差异表达。
方法
进行了一项病例对照研究，纳入125例甲状腺乳头状癌（PTC）患者和125例对照。采用四引物扩增阻滞突变系统-聚合酶链反应（ARMS-PCR）和PCR-限制性片段长度多态性（PCR-RFLP）方法对多态性进行基因分型。
结果
在GAS-5 145204276和MEG3 rs4081134多态性的基因型和等位基因频率方面，两组之间未发现显著关联。遗传模型也显示了相同的结果。关于NEAT1 rs512715，PTC组的GC基因型和NEAT1 rs512715的超显性模型比对照组更多，而对照组的隐性模型频率更高。
结论
GAS5 rs145204276和MEG3 rs4081134多态性与甲状腺乳头状癌（PTC）风险无显著关联。相比之下，NEAT1 rs512715对PTC的发生有显著影响。
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