Chen Hongyu, Zhang Jinjin, Ren Jinwei, Wang Wenzhao, Xiong Weiping, Zhang Yaodong, Bao Li, Liu Hongwei
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichenxi Road, Chaoyang District, Beijing, 100101, P. R. China.
Savaid Medical School, University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
Chem Biodivers. 2018 May;15(5):e1700567. doi: 10.1002/cbdv.201700567. Epub 2018 Apr 29.
Ganoderma leucocontextum is a well-known medicinal mushroom cultivated in the Tibet Plateau of China. Chemistry investigation on the fruiting bodies of this mushroom resulted in the isolation of sixteen secondary metabolites including three new lanostane triterpenes, ganoleucoins Q - S (1 - 3), as well as thirteen known compounds (4 - 16). The structures of compounds 1 - 3 were determined by NMR, MS, CD spectral analysis, and chemical derivation method. The neuroprotective effects of compounds 1 - 16 were tested on PC12 cells. Compounds 1 and 2 showed protective effects against the H O induced damage with the survival rate of 83.19 ± 0.92%, 73.37 ± 1.25% at the concentration of 200 μm, respectively. Meanwhile, compounds 1 and 2 induced neurite outgrowth at 50 - 200 μm. The results from this study suggested that G. leucocontextum and its metabolites may be potential functional food ingredients for the prevention of neurodegenerative diseases.
白肉灵芝是中国青藏高原栽培的一种著名药用真菌。对该蘑菇子实体进行化学研究，从中分离出16种次生代谢产物，包括3种新的羊毛甾烷三萜类化合物，即白肉灵芝素Q - S（1 - 3），以及13种已知化合物（4 - 16）。通过核磁共振（NMR）、质谱（MS）、圆二色光谱（CD）分析和化学衍生方法确定了化合物1 - 3的结构。在PC12细胞上测试了化合物1 - 16的神经保护作用。化合物1和2对过氧化氢诱导的损伤具有保护作用，在浓度为200 μM时，存活率分别为83.19 ± 0.92%、73.37 ± 1.25%。同时，化合物1和2在50 - 200 μM时可诱导神经突生长。本研究结果表明，白肉灵芝及其代谢产物可能是预防神经退行性疾病的潜在功能性食品成分。
