Laboratory for Genome Engineering, Division of Biological Sciences, 4700 King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia.
Laboratory for Genome Engineering, Division of Biological Sciences, 4700 King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia.
Trends Plant Sci. 2018 May;23(5):374-378. doi: 10.1016/j.tplants.2018.03.003. Epub 2018 Mar 28.
Almost all biological processes involve RNA, making it crucial to develop tools for manipulation of the transcriptome. The bacterial CRISPR/Cas13 system was recently rewired to facilitate RNA manipulation in eukaryotes, including plants. We discuss here the opportunities and limitations of using CRISPR/Cas13 in plants for various types of RNA manipulation.
几乎所有的生物过程都涉及 RNA，因此开发用于转录组操作的工具至关重要。细菌的 CRISPR/Cas13 系统最近被重新布线，以促进真核生物（包括植物）中的 RNA 操作。我们在这里讨论了在植物中使用 CRISPR/Cas13 进行各种类型的 RNA 操作的机会和限制。
