在梅加拉亚邦牛群的牛血清样本中检测经典猪瘟病毒E2基因。
Detection of classical swine fever virus E2 gene in cattle serum samples from cattle herds of Meghalaya.
作者信息
Chakraborty A K, Karam A, Mukherjee P, Barkalita L, Borah P, Das S, Sanjukta R, Puro K, Ghatak S, Shakuntala I, Sharma I, Laha R G, Sen A
机构信息
Division of Animal Health, ICAR RC for NEH Region, Umiam, Meghalaya India.
2Department of Microbiology, Assam University, Silchar, Assam India.
出版信息
Virusdisease. 2018 Mar;29(1):89-95. doi: 10.1007/s13337-018-0433-9. Epub 2018 Feb 17.
The present study focused on the detection and genetic characterisation of 5' untranslated region (5'UTR) and E2 gene of classical swine fever virus (CSFV, family , genus ) from bovine population of the northeastern region of India. A total of 134 cattle serum samples were collected from organised cattle farms and were screened for CSFV antigen with a commercial antigen capture enzyme linked immunosorbent assay (Ag-ELISA) and reverse transcription-polymerase chain reaction (RT-PCR). A total of 10 samples were positive for CSFV antigen by ELISA, while all of them were positive in PCR for 5'UTR region. Full length E2 region of CSFV were successfully amplified from two positive samples and used for subsequent phylogenetic analysis and determination of protein 3D structure which showed similarity with reported CSFV isolate from Assam of sub-genogroup 2.1, with minor variations in protein structure.
本研究聚焦于从印度东北地区的牛群中检测经典猪瘟病毒（CSFV，科 ，属 ）的5'非翻译区（5'UTR）和E2基因，并对其进行遗传特征分析。从规模化养牛场共采集了134份牛血清样本，采用商业抗原捕获酶联免疫吸附测定（Ag-ELISA）和逆转录聚合酶链反应（RT-PCR）对CSFV抗原进行筛查。通过ELISA检测，共有10份样本CSFV抗原呈阳性，而所有样本在5'UTR区域的PCR检测中均为阳性。从两份阳性样本中成功扩增出CSFV的全长E2区域，并用于后续的系统发育分析和蛋白质3D结构测定，结果显示其与来自阿萨姆邦的2.1亚基因组报道的CSFV分离株相似，蛋白质结构存在微小差异。
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