米佐拉姆邦疫情中涉及的经典猪瘟病毒的分子特征分析
Molecular Characterization of Classical swine fever virus Involved in the Outbreak in Mizoram.
作者信息
Barman N N, Gupt R S, Bora D P, Kataria R S, Tiwari A K, Roychoudhury P
机构信息
Department of Microbiology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, 781 022 Assam India.
出版信息
Indian J Virol. 2010 Jun;21(1):76-81. doi: 10.1007/s13337-010-0006-z. Epub 2010 Sep 22.
Classical swine fever is the most insidious and devastating disease of pigs and wild boars. The virus is closely related to the other members of the genus Pestivirus. The outbreak recorded in Mizoram, India was strategically important as the state shares porous international boundary with East Asian countries. Both immunodiagnostic and molecular techniques were used to confirm the involvement of Classical swine fever virus (CSFV) in this outbreak. Sandwich ELISA and direct FAT could detect CSFV in the tissue samples. RT-nPCR specifically amplified E2 and 5'NTR product of 271 bp. Phylogenetic analysis showed, that the Mizoram isolate (MZ4/69) was very close to the Chinese strain Shimen-HVRI (93.0%) rather than other Indian isolate (CSF-30-03). Present study provides a valuable sequence based molecular data on Indian isolate of CSFV and will be useful in investigation on transmission of such disease from neighbour countries.
经典猪瘟是猪和野猪中最隐匿且具毁灭性的疾病。该病毒与瘟病毒属的其他成员密切相关。印度米佐拉姆邦记录的疫情具有重要战略意义，因为该邦与东亚国家接壤，边境管控较为薄弱。免疫诊断和分子技术均被用于确认经典猪瘟病毒（CSFV）是否参与此次疫情。夹心ELISA和直接荧光抗体试验（FAT）能够在组织样本中检测到CSFV。逆转录巢式聚合酶链反应（RT-nPCR）特异性扩增出271bp的E2和5'非翻译区（NTR）产物。系统发育分析表明，米佐拉姆邦分离株（MZ4/69）与中国石门-HVRI株非常接近（相似度为93.0%），而非其他印度分离株（CSF-30-03）。本研究提供了基于序列的有关印度CSFV分离株的宝贵分子数据，将有助于调查此类疾病从邻国的传播情况。
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