Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine.
Department of Infection Metagenomics, Research Institute for Microbial Diseases, Osaka University.
Circ J. 2018 May 25;82(6):1640-1650. doi: 10.1253/circj.CJ-17-1285. Epub 2018 Mar 30.
Research suggests that heart failure with reduced ejection fraction (HFrEF) is a state of systemic inflammation that may be triggered by microbial products passing into the bloodstream through a compromised intestinal barrier. However, whether the intestinal microbiota exhibits dysbiosis in HFrEF patients is largely unknown.
Twenty eight non-ischemic HFrEF patients and 19 healthy controls were assessed by 16S rRNA analysis of bacterial DNA extracted from stool samples. After processing of sequencing data, bacteria were taxonomically classified, diversity indices were used to examine microbial ecology, and relative abundances of common core genera were compared between groups. Furthermore, we predicted gene carriage for bacterial metabolic pathways and inferred microbial interaction networks on multiple taxonomic levels.Bacterial communities of both groups were dominated by the Firmicutes and Bacteroidetes phyla. The most abundant genus in both groups wasBacteroides. Although α diversity did not differ between groups, ordination by β diversity metrics revealed a separation of the groups across components of variation.StreptococcusandVeillonellawere enriched in the common core microbiota of patients, whileSMB53was depleted. Gene families in amino acid, carbohydrate, vitamin, and xenobiotic metabolism showed significant differences between groups. Interaction networks revealed a higher degree of correlations between bacteria in patients.
Non-ischemic HFrEF patients exhibited multidimensional differences in intestinal microbial communities compared with healthy subjects.
研究表明，射血分数降低的心力衰竭（HFrEF）是一种全身炎症状态，可能是由于肠道屏障受损，微生物产物进入血液而引发的。然而，HFrEF 患者的肠道微生物群是否存在失调尚不清楚。
对 28 名非缺血性 HFrEF 患者和 19 名健康对照者的粪便样本中的细菌 DNA 进行 16S rRNA 分析。在处理测序数据后，对细菌进行分类学分类，使用多样性指数来检查微生物生态，并比较两组之间常见核心属的相对丰度。此外，我们预测了细菌代谢途径的基因携带情况，并在多个分类水平上推断了微生物相互作用网络。两组的细菌群落均以厚壁菌门和拟杆菌门为主。两组中最丰富的属均为拟杆菌属。尽管两组之间的α多样性没有差异，但基于β多样性指标的排序显示两组在变异成分上存在分离。患者常见核心微生物群中富集了链球菌属和韦荣球菌属，而 SMB53 则被消耗。氨基酸、碳水化合物、维生素和外源性代谢相关的基因家族在两组间存在显著差异。相互作用网络显示患者体内细菌之间的相关性更高。
与健康受试者相比，非缺血性 HFrEF 患者的肠道微生物群落表现出多维差异。
