Kamo Takehiro, Akazawa Hiroshi, Suda Wataru, Saga-Kamo Akiko, Shimizu Yu, Yagi Hiroki, Liu Qing, Nomura Seitaro, Naito Atsuhiko T, Takeda Norifumi, Harada Mutsuo, Toko Haruhiro, Kumagai Hidetoshi, Ikeda Yuichi, Takimoto Eiki, Suzuki Jun-Ichi, Honda Kenya, Morita Hidetoshi, Hattori Masahira, Komuro Issei
Department of Cardiovascular Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Japan.
PLoS One. 2017 Mar 22;12(3):e0174099. doi: 10.1371/journal.pone.0174099. eCollection 2017.
Emerging evidence has suggested a potential impact of gut microbiota on the pathophysiology of heart failure (HF). However, it is still unknown whether HF is associated with dysbiosis in gut microbiota. We investigated the composition of gut microbiota in patients with HF to elucidate whether gut microbial dysbiosis is associated with HF. We performed 16S ribosomal RNA gene sequencing of fecal samples obtained from 12 HF patients and 12 age-matched healthy control (HC) subjects, and analyzed the differences in gut microbiota. We further compared the composition of gut microbiota of 12 HF patients younger than 60 years of age with that of 10 HF patients 60 years of age or older. The composition of gut microbial communities of HF patients was distinct from that of HC subjects in both unweighted and weighted UniFrac analyses. Eubacterium rectale and Dorea longicatena were less abundant in the gut microbiota of HF patients than in that of HC subjects. Compared to younger HF patients, older HF patients had diminished proportions of Bacteroidetes and larger quantities of Proteobacteria. The genus Faecalibacterium was depleted, while Lactobacillus was enriched in the gut microbiota of older HF patients. These results suggest that patients with HF harbor significantly altered gut microbiota, which varies further according to age. New concept of heart-gut axis has a great potential for breakthroughs in the development of novel diagnostic and therapeutic approach for HF.
新出现的证据表明肠道微生物群对心力衰竭（HF）的病理生理学可能产生影响。然而，HF是否与肠道微生物群失调相关仍不清楚。我们调查了HF患者的肠道微生物群组成，以阐明肠道微生物失调是否与HF相关。我们对从12例HF患者和12例年龄匹配的健康对照（HC）受试者获得的粪便样本进行了16S核糖体RNA基因测序，并分析了肠道微生物群的差异。我们进一步比较了12例年龄小于60岁的HF患者与10例年龄60岁及以上的HF患者的肠道微生物群组成。在未加权和加权UniFrac分析中，HF患者的肠道微生物群落组成与HC受试者不同。直肠真杆菌和长链多尔氏菌在HF患者肠道微生物群中的丰度低于HC受试者。与年轻HF患者相比，老年HF患者拟杆菌比例降低，变形菌数量增加。在老年HF患者的肠道微生物群中，粪杆菌属减少，而乳酸杆菌属富集。这些结果表明，HF患者的肠道微生物群有显著改变，且随年龄进一步变化。心-肠轴的新概念在HF新型诊断和治疗方法的开发方面具有巨大的突破潜力。
