对具有非化学计量O-乙酰化和非化学计量侧链L-岩藻糖基化特征的阿尔伯特埃希氏菌O2 O-多糖的研究。
Studies on the O-polysaccharide of Escherichia albertii O2 characterized by non-stoichiometric O-acetylation and non-stoichiometric side-chain l-fucosylation.
作者信息
Naumenko Olesya I, Zheng Han, Xiong Yanwen, Senchenkova Sof'ya N, Wang Hong, Shashkov Alexander S, Li Qun, Wang Jianping, Knirel Yuriy A
机构信息
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia; Higher Chemical College of the Russian Academy of Sciences, D. I. Mendeleev University of Chemical Technology of Russia, Moscow, Russia.
State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, China.
出版信息
Carbohydr Res. 2018 May 22;461:80-84. doi: 10.1016/j.carres.2018.02.013. Epub 2018 Mar 6.
An O-polysaccharide was isolated from the lipopolysaccharide of Escherichia albertii O2 and studied by chemical methods and 1D and 2D H and C NMR spectroscopy. The following structure of the O-polysaccharide was established: . The O-polysaccharide is characterized by masked regularity owing to a non-stoichiometric O-acetylation of an l-fucose residue in the main chain and a non-stoichiometric side-chain l-fucosylation of a β-GlcNAc residue. A regular linear polysaccharide was obtained by sequential Smith degradation and alkaline O-deacetylation of the O-polysaccharide. The content of the O-antigen gene cluster of E. albertii O2 was found to be essentially consistent with the O-polysaccharide structure established.
从艾氏埃希菌O2的脂多糖中分离出一种O-多糖，并通过化学方法以及一维和二维氢谱和碳谱核磁共振波谱进行研究。确定了该O-多糖的以下结构：。由于主链中L-岩藻糖残基的非化学计量O-乙酰化以及β-GlcNAc残基的非化学计量侧链L-岩藻糖基化，该O-多糖具有隐蔽的规律性。通过对该O-多糖进行连续的史密斯降解和碱性O-脱乙酰化，得到了一种规则的线性多糖。发现艾氏埃希菌O2的O-抗原基因簇的含量与所确定的O-多糖结构基本一致。
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