高滴度逆转录病毒和慢病毒载体的生产。
Production of High-Titer Retrovirus and Lentivirus Vectors.
作者信息
Sena-Esteves Miguel, Gao Guangping
出版信息
Cold Spring Harb Protoc. 2018 Apr 2;2018(4):2018/4/pdb.prot095687. doi: 10.1101/pdb.prot095687.
PMID:29610362
Abstract
The most commonly used method for production of retrovirus and lentivirus vectors in research laboratories is of transient transfection of 293T cells with a lentivirus vector plasmid, packaging genome plasmid(s), and an envelope expression plasmid. High-titer stocks are obtained by concentration of vector supernatants by ultracentrifugation.
摘要
在研究实验室中，生产逆转录病毒和慢病毒载体最常用的方法是用慢病毒载体质粒、包装基因组质粒和包膜表达质粒瞬时转染293T细胞。通过超速离心浓缩载体上清液可获得高滴度病毒储存液。
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