一种改良的慢病毒载体生产方案。
An Improved Protocol for the Production of Lentiviral Vectors.
机构信息
Duke Viral Vector Core, Department of Neurobiology, Duke University Medical Center, Durham, NC 27710, USA.
CLAIRIgene Inc., Durham, NC 27701, USA.
出版信息
STAR Protoc. 2020 Oct 27;1(3):100152. doi: 10.1016/j.xpro.2020.100152. eCollection 2020 Dec 18.
Abstract
Lentiviral vectors are an ideal gene-delivery system for large gene-editing tools, such as the clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 system, due to their high packaging capacity and broad tropism. Here, we present a calcium phosphate-based protocol for lentiviral production and concentration for and use. This revised procedure has been optimized to ensure high viral titers and transduction efficiency and is scalable to meet specific production needs.
摘要
慢病毒载体因其高包装容量和广泛的嗜性，是包括 CRISPR-Cas9 系统在内的大型基因编辑工具的理想基因传递系统。本文介绍了一种基于磷酸钙的慢病毒生产和浓缩方法，适用于 和 实验。该改进后的方案经过优化，可确保高病毒滴度和转导效率，并可根据特定的生产需求进行扩展。
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