Centre for Eye Health, University of New South Wales, New South Wales, Australia.
School of Optometry and Vision Science, University of New South Wales, New South Wales, Australia.
Invest Ophthalmol Vis Sci. 2018 Apr 1;59(5):1693-1703. doi: 10.1167/iovs.17-23683.
To investigate the effect of stimulus size and disease status on the structure-function relationship within the central retina, we correlated the differential light sensitivity (DLS) with Goldmann stimulus size I to V (GI-V) and optical coherence tomography (OCT) derived in vivo ganglion cell count per stimulus area (GCc) within the macular area in normal subjects and patients with early glaucoma.
Humphrey Field Analyzer 10-2 visual field data with GI through V and Spectralis OCT macular ganglion cell layer (GCL) thickness measurements were collected from normal and early glaucoma cohorts including 25 subjects each. GCc was calculated from GCL thickness data and correlated with DLSs for different stimulus sizes.
Correlation coefficients attained with smaller stimulus size were higher compared to larger stimulus sizes in both normal (GI-GII: R2 = 0.41-0.43, GIII-GV: R2 = 0.16-0.41) and diseased cohorts (GI-GII: R2 = 0.33-0.41, GIII-GV: R2 = 0.19-0.36). Quadratic regression curves for combined GI to V data demonstrated high correlation (R2= 0.82-0.90) and differed less than 1 dB of visual sensitivity within the GCc range between cohorts. The established structure-function relationship was compatible with a histologically derived model correlation spanning the range predicted by stimulus sizes GI to GIII.
Stimulus sizes within critical spatial summation area (GI-II) improved structure-function correlations in the central visual field. The structure-function relationship was identical in both normal and diseased cohort when GI to GV data were combined. Congruency of GI and GII structure-function correlation with those previously derived with GIII from more peripheral locations further suggests that the structure-function relationship is governed by the number of ganglion cell per stimulus area.
为了研究刺激大小和疾病状态对中心视网膜结构-功能关系的影响，我们将差分光灵敏度（DLS）与Goldmann 刺激大小 I 到 V（GI-V）以及在正常受试者和早期青光眼患者的黄斑区的活体神经节细胞计数（GCc）进行了相关性分析。
从正常和早期青光眼队列中收集了 25 名受试者的 Humphrey Field Analyzer 10-2 视野数据，包括 GI 至 V 以及 Spectralis OCT 黄斑神经节细胞层（GCL）厚度测量。从 GCL 厚度数据中计算 GCc，并与不同刺激大小的 DLS 相关联。
在正常（GI-GII：R2 = 0.41-0.43，GIII-GV：R2 = 0.16-0.41）和患病（GI-GII：R2 = 0.33-0.41，GIII-GV：R2 = 0.19-0.36）队列中，与较大的刺激大小相比，较小刺激大小的相关系数更高。对于组合的 GI 到 V 数据，二次回归曲线显示出高度相关性（R2 = 0.82-0.90），并且在 GCc 范围内，不同队列之间的视觉灵敏度差异小于 1 dB。建立的结构-功能关系与组织学衍生的模型相关性一致，该相关性跨越了从刺激大小 GI 到 GIII 预测的范围。
在中央视野中，临界空间总和区域（GI-II）内的刺激大小提高了结构-功能相关性。当组合 GI 到 GV 数据时，正常和患病队列的结构-功能关系是相同的。GI 和 GII 结构-功能相关性与以前从更外围位置用 GIII 得出的相关性一致，这进一步表明结构-功能关系由每个刺激区域的神经节细胞数量决定。
