Center for Research in Advanced Materials (CIMAV S.C.), Miguel de Cervantes 120, 31136 Chihuahua, Mexico.
CIMAV S.C., Ejido Arroyo Seco, 34147 Durango, Mexico.
Sensors (Basel). 2018 Apr 3;18(4):1071. doi: 10.3390/s18041071.
The high incidence of Diabetes Mellitus in low-income regions has promoted the development of low-cost alternatives to replace blood-based procedures. In this work, we present a bienzymatic paper-based sensor suitable for the naked-eye detection of glucose in saliva samples. The sensor was obtained by a stamping procedure and modified with chitosan to improve the colorimetric readout. The bienzymatic reaction of GOx-HRP coupled with 2,4,6-tribromo-3-hydroxy benzoic acid was applied for the detection of glucose within a range from 0 to 180 mgdL in buffer and artificial saliva solutions. The visual readout was perceived by the naked eye and registered with an office scanner to evaluate the analytical performance. The results showed a limit of detection of 0.37 mgdL (S/N = 3) with an R.S.D. of 1.69% and a linear range from 1 to 22.5 mgdL with an R² of 0.99235. The analysis of human saliva samples was performed without pre-processing, achieving recoveries from 92 to 114%. The naked-eye detection was evaluated under two different light settings, showing average recoveries of 108.58 and 90.65% for standard and low illumination. The proposed device showed potential for easy-to-use, sensitive, low-cost, fast, and device-free detection of salivary glucose suitable for untrained personnel operation and limited facilities.
低收入地区糖尿病发病率高，推动了低成本替代品的发展，以替代基于血液的检测方法。在这项工作中，我们提出了一种适用于通过肉眼检测唾液样本中葡萄糖的双酶纸基传感器。该传感器通过压印工艺获得，并通过壳聚糖进行修饰，以改善比色读数。GOx-HRP 的双酶反应与 2,4,6-三溴-3-羟基苯甲酸偶联，用于在缓冲液和人工唾液溶液中检测 0 至 180mgdL 范围内的葡萄糖。通过肉眼感知可视读数，并使用办公扫描仪进行记录，以评估分析性能。结果表明，在缓冲液和人工唾液溶液中，检测限为 0.37mgdL（S/N=3），相对标准偏差为 1.69%，线性范围为 1 至 22.5mgdL，相关系数为 0.99235。对人唾液样本进行了无需预处理的分析，回收率为 92%至 114%。在两种不同的光照条件下对肉眼检测进行了评估，标准光照和低光照下的平均回收率分别为 108.58%和 90.65%。该设备具有易于使用、灵敏度高、成本低、快速和无需设备等优点，适合未经训练的人员操作和有限的设施。
