Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
Molecules. 2019 Aug 22;24(17):3052. doi: 10.3390/molecules24173052.
An efficient microwave-assisted extraction (MAE) combined with in-capillary [Fe(ferrozine)3]2+-capillary electrophoresis-Diode Array Detector (in-capillary [Fe(ferrozine)3]2+-CE-DAD) was developed to screen active components with the ability to chelate ferrous ions and determine the total antioxidant activity. The MAE conditions, including methanol concentration, extraction power, extraction time, and the ratio of material to liquid, were optimized by an L(3) orthogonal experiment. Background buffer, voltage, and cartridge temperature that affect the separation of six compounds were optimized. It was found that rutin and quercetin were the main components chelating ferrous ions in Flos Sophorae Immaturus (Flos Sophorae) by the in-capillary [Fe(ferrozine)]-CE-DAD. The recoveries were ranged from 95.2% to 104%. It was concluded that the MAE combined with in-capillary [Fe(ferrozine)]-CE-DAD method was a simple, reliable, and efficient tool for screening active components from the complex traditional Chinese medicine samples and evaluating their ability to chelate ferrous ions.
建立了一种高效的微波辅助提取（MAE）结合毛细管内[Fe（ferrozine）3]2+-毛细管电泳-二极管阵列检测器（in-capillary [Fe（ferrozine）3]2+-CE-DAD），用于筛选具有螯合亚铁离子能力的活性成分并测定总抗氧化活性。通过 L(3)正交实验优化了 MAE 条件，包括甲醇浓度、提取功率、提取时间和料液比。优化了影响六种化合物分离的背景缓冲液、电压和卡盒温度。发现芦丁和槲皮素是槐米（Flos Sophorae）中螯合亚铁离子的主要成分。通过毛细管内[Fe（ferrozine）]-CE-DAD 测定，回收率在 95.2%至 104%之间。结论：MAE 结合毛细管内[Fe（ferrozine）]-CE-DAD 方法是一种简单、可靠、高效的工具，可用于从复杂的中药样品中筛选活性成分，并评估其螯合亚铁离子的能力。
