Department of Cogno-Mechatronics Engineering, College of Nanoscience & Nanotechnology, Pusan National University, Busan, 46241, Republic of Korea.
Research Center for Energy Convergence Technology, Pusan National University, Busan, 46241, Republic of Korea.
Sci Rep. 2018 Apr 3;8(1):5570. doi: 10.1038/s41598-018-23958-3.
Neural tissue regeneration is a significant challenge, because severe nerve injury is quite difficult to regenerate spontaneously. Although, many studies have been devoted to promote nerve regeneration, there are still many technical challenges to achieve satisfactory results. In this study, we designed biomimetic matrices composed of aligned laminin core-polydioxanone/collagen shell (Lam-PDO/Col) fibers, which can provide both topographical and biochemical cues for promoting neuritogenesis. The aligned Lam-PDO/Col core-shell fiber matrices were fabricated by magnetic field-assisted electrospinning with the coaxial system, and their potential as biofunctional scaffolds for promoting neuritogenesis was explored. It was demonstrated that the aligned Lam-PDO/Col core-shell fibers were successfully fabricated, and the laminin in the core of fibers was steadily and continuously released from fibers. In addition, the cellular behaviors of hippocampal neuronal cells on the matrices were significantly enhanced. Moreover, the aligned Lam-PDO/Col fiber matrices effectively improved and guided neurite outgrowth as well as the neurogenic differentiation by providing both topographical and biochemical cues through aligned fiber structure and sustained release of laminin. Collectively, it is suggested that the aligned Lam-PDO/Col core-shell fiber matrices are one of the most promising approaches for promoting neuritogenesis and neural tissue regeneration.
神经组织再生是一个重大的挑战，因为严重的神经损伤很难自发再生。尽管许多研究致力于促进神经再生，但仍有许多技术挑战需要克服，才能取得满意的效果。在这项研究中，我们设计了由取向的层粘连蛋白核心-聚二氧杂环己酮/胶原蛋白壳（Lam-PDO/Col）纤维组成的仿生基质，它可以提供促进神经突形成的形态和生化线索。通过同轴系统的磁场辅助静电纺丝成功制备了取向的 Lam-PDO/Col 核壳纤维基质，并探讨了其作为促进神经突形成的生物功能支架的潜力。结果表明，成功制备了取向的 Lam-PDO/Col 核壳纤维，纤维内部的层粘连蛋白可以从纤维中稳定且持续地释放。此外，基质上海马神经元细胞的细胞行为得到显著增强。此外，通过取向纤维结构和层粘连蛋白的持续释放，提供形态和生化线索，取向的 Lam-PDO/Col 纤维基质有效地促进和引导神经突生长和神经发生分化。综上所述，取向的 Lam-PDO/Col 核壳纤维基质是促进神经突形成和神经组织再生的最有前途的方法之一。
