Pietrangelo Tiziana, Di Filippo Ester S, Locatelli Marcello, Piacenza Francesco, Farina Marco, Pavoni Eleonora, Di Donato Andrea, Innosa Denise, Provinciali Mauro, Fulle Stefania
Dipartimento Neuroscienze Imaging and Scienze Cliniche, Università degli Studi "G. d'Annunzio" Chieti-Pescara, Chieti, Italy.
Dipartimento di Farmacia, Università degli Studi "G. d'Annunzio" Chieti-Pescara, Chieti, Italy.
Front Pharmacol. 2018 Mar 16;9:152. doi: 10.3389/fphar.2018.00152. eCollection 2018.
The extracellular guanosine 5'-triphosphate, GTP, has been demonstrated to be an enhancer of myogenic cell differentiation in a murine cell line, not yet in human muscle cells. Our hypothesis was that GTP could influence also human skeletal muscle regeneration, specifically in the first phases. We tested GTP stimulus on human muscle precursor cells established in culture by human satellite cells derived from Vastus Lateralis of three young male. Our data show that extracellular GTP (a) up-regulated miRNA (specifically miR133a and miR133b) and myogenic regulator factor and (b) induces human myogenic precursor cells to release exosomes stuffed with guanosine based molecules (mainly guanosine) in the extracellular milieu. We think that probably these exosomes could be addressed to influence by means of their content (mainly guanosine) in paracrine or autocrine manner the surrounding cells and/or at distance other muscles or tissues.
细胞外鸟苷 5'-三磷酸（GTP）已被证明是小鼠细胞系中成肌细胞分化的增强剂，但尚未在人类肌肉细胞中得到证实。我们的假设是，GTP 也可能影响人类骨骼肌再生，特别是在最初阶段。我们用来自三名年轻男性股外侧肌的人卫星细胞在培养中建立的人类肌肉前体细胞测试了 GTP 刺激。我们的数据表明，细胞外 GTP（a）上调了 miRNA（特别是 miR133a 和 miR133b）和成肌调节因子，并且（b）诱导人类成肌前体细胞在细胞外环境中释放充满鸟苷类分子（主要是鸟苷）的外泌体。我们认为，这些外泌体可能通过其内容物（主要是鸟苷）以旁分泌或自分泌方式影响周围细胞和/或远处的其他肌肉或组织。
