Yang Xiping, Islam Md S, Sood Sushma, Maya Stephanie, Hanson Erik A, Comstock Jack, Wang Jianping
Department of Agronomy, Genetics Institute, University of Florida, Gainesville, FL, United States.
Sugarcane Field Station, United States Department of Agriculture-Agricultural Research Service, Canal Point, FL, United States.
Front Plant Sci. 2018 Mar 19;9:350. doi: 10.3389/fpls.2018.00350. eCollection 2018.
Sugarcane ( spp.) is an important economic crop, contributing up to 80% of table sugar used in the world and has become a promising feedstock for biofuel production. Sugarcane production has been threatened by many diseases, and fungicide applications for disease control have been opted out for sustainable agriculture. Orange rust is one of the major diseases impacting sugarcane production worldwide. Identifying quantitative trait loci (QTLs) and developing diagnostic markers are valuable for breeding programs to expedite release of superior sugarcane cultivars for disease control. In this study, an F segregating population derived from a cross between two hybrid sugarcane clones, CP95-1039 and CP88-1762, was evaluated for orange rust resistance in replicated trails. Three QTLs controlling orange rust resistance in sugarcane (qORR109, qORR4 and qORR102) were identified for the first time ever, which can explain 58, 12 and 8% of the phenotypic variation, separately. We also characterized 1,574 sugarcane putative resistance () genes. These sugarcane putative genes and simple sequence repeats in the QTL intervals were further used to develop diagnostic markers for marker-assisted selection of orange rust resistance. A PCR-based gene-derived maker, G1 was developed, which showed significant association with orange rust resistance. The putative QTLs and marker developed in this study can be effectively utilized in sugarcane breeding programs to facilitate the selection process, thus contributing to the sustainable agriculture for orange rust disease control.
甘蔗（甘蔗属）是一种重要的经济作物，提供了全球80%的食用糖，并且已成为生物燃料生产中一种很有前景的原料。甘蔗生产受到多种病害的威胁，为了可持续农业，已不再选择使用杀菌剂来控制病害。橙锈病是影响全球甘蔗生产的主要病害之一。鉴定数量性状位点（QTL）并开发诊断标记对于育种计划很有价值，有助于加快释放用于病害控制的优良甘蔗品种。在本研究中，对由两个杂交甘蔗克隆CP95 - 1039和CP88 - 1762杂交产生的F分离群体在重复试验中进行了橙锈病抗性评估。首次鉴定出三个控制甘蔗橙锈病抗性的QTL（qORR109、qORR4和qORR102），它们分别可解释58%、12%和8%的表型变异。我们还对1574个甘蔗假定抗性（）基因进行了表征。这些甘蔗假定基因和QTL区间内的简单序列重复进一步用于开发诊断标记，以辅助选择抗橙锈病。开发了一种基于PCR的基因衍生标记G1，它与橙锈病抗性显著相关。本研究中鉴定的假定QTL和开发的标记可有效地用于甘蔗育种计划，以促进选择过程，从而有助于可持续农业控制橙锈病。
