Bãicuş Anda
Roum Arch Microbiol Immunol. 2016 Jan-Jun;75(1-2):52-4.
Environmental enterovirus surveillance plays a key role in the detection and identification of importation of circulating vaccine-derived polioviruses into polio-free areas. In 2015, 2 cases of paralytic poliomyelitis caused by circulating vaccine-derived poliovirus type 1 (cVDPV1), from south-western Ukraine, bordering Romania, were confirmed. Romania was considered a country at risk and the environmental enterovirus surveillance was enhanced. In this context we tried to find a diagnosis algorithm for the rapid detection of poliovirus (PV) in the sewage water, using a combination between a rapid molecular method for human enterovirus (HEV) detection using the GeneXpert system and the virus isolation on cell culture lines. By using this algorithm, we would be able to give a rapid response in an emergency situation, such as the risk of polio importation.
环境肠道病毒监测在检测和识别疫苗衍生脊髓灰质炎病毒输入无脊髓灰质炎地区方面发挥着关键作用。2015年，确诊了2例由1型疫苗衍生脊髓灰质炎病毒（cVDPV1）引起的麻痹性脊髓灰质炎病例，病例来自与罗马尼亚接壤的乌克兰西南部。罗马尼亚被视为有风险的国家，因此加强了环境肠道病毒监测。在此背景下，我们试图找到一种诊断算法，用于快速检测污水中的脊髓灰质炎病毒（PV），该算法结合了使用GeneXpert系统快速检测人肠道病毒（HEV）的分子方法和在细胞系上进行病毒分离。通过使用该算法，我们将能够在紧急情况下，如脊髓灰质炎输入风险时做出快速反应。
