利用细胞培养和分子方法检测污水中的脊髓灰质炎病毒。
Detection of Polioviruses in Sewage Using Cell Culture and Molecular Methods.
作者信息
Figas Agnieszka, Wieczorek Magdalena, Litwińska Bogumiła, Gut Włodzimierz
机构信息
National Institute of Public Health - National Institute of Hygiene, Department of Virology, Warsaw, Poland.
出版信息
Pol J Microbiol. 2017 Jan 2;65(4):479-483. doi: 10.5604/17331331.1227676.
The work presented here demonstrates the utility of a two-step algorithm for environmental poliovirus surveillance based on: preselection of sewage samples tested for the presence of enteroviral genetic material-RT-PCR assay and detection of infectious viruses by cell culture technique (L20B for polioviruses and RD for polio and other non-polio enteroviruses). RD and L20B cell lines were tested to determine their sensitivity for isolation of viruses from environmental samples (sewage). Finally, we wanted to determine if sewage concentration affects the results obtained for RT-PCR and cell cultures.
本文展示了一种基于两步算法的环境脊髓灰质炎病毒监测方法的实用性，该方法包括：对检测肠道病毒遗传物质的污水样本进行预筛选——逆转录聚合酶链反应（RT-PCR）检测，并通过细胞培养技术检测传染性病毒（脊髓灰质炎病毒用L20B细胞系，脊髓灰质炎病毒及其他非脊髓灰质炎肠道病毒用RD细胞系）。对RD和L20B细胞系进行了测试，以确定它们从环境样本（污水）中分离病毒的敏感性。最后，我们想确定污水浓缩是否会影响RT-PCR和细胞培养的结果。
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