分别从生牛乳中分离得到的新种及新种。

sp. nov and sp. nov., isolated from raw cow's milk.

机构信息

University of Bonn, Institute of Nutritional and Food Science, Food Microbiology and Hygiene, Friedrich-Hirzebruch-Allee 7, 53115 Bonn, Germany.

GFZ German Research Centre for Geosciences, Section Geomicrobiology, Potsdam, Germany.

出版信息

Int J Syst Evol Microbiol. 2023 Oct;73(10). doi: 10.1099/ijsem.0.006141.

DOI:10.1099/ijsem.0.006141

PMID:37889136

Abstract

Four Gram-positive, rod-shaped, none-sporeforming, non-motile isolates were obtained from various raw milk samples taken from the cooling tank on a research farm in Königswinter, Germany. Based on phylogenetic analysis of the 16S rRNA genes and whole genome sequences, all isolates were assigned to the genus , but were divided in two different groups. All isolates contained C 9 and C as predominant fatty acids, as well as traces of C. They all contained menaquinones MK-8 (H) and MK-9 (H) and produced mycolic acids characteristic for the majority of species belonging to the genus . 16S rRNA gene sequence similarity values to the closest related type strains DSM 45392 and DSM 20521 were below 98.7 %, average nucleotide identity values were below 86 % and digital DNA-DNA-hybridization values were below 25 %, indicating that the isolates represent two novel species. The names sp. nov. and sp. nov. are proposed, represented by the type strains LM112 (=DSM 116216=HAMBI 3782) and R4 (=DSM 116183=HAMBI 3785), respectively.

摘要

从德国 Königswinter 研究农场冷却罐中的各种生牛乳样本中获得了四株革兰氏阳性、杆状、无芽孢、非运动性的分离株。基于 16S rRNA 基因和全基因组序列的系统发育分析，所有分离株均被分配到属，但分为两个不同的组。所有分离株均含有 C 9 和 C 作为主要脂肪酸，以及痕量的 C 。它们都含有menaquinones MK-8 (H) 和 MK-9 (H)，并产生属于属的大多数种所特有的分枝菌酸。与最接近的参考菌株 DSM 45392 和 DSM 20521 的 16S rRNA 基因序列相似性值低于 98.7%，平均核苷酸同一性值低于 86%，数字 DNA-DNA 杂交值低于 25%，表明这些分离株代表了两个新的种。提议使用代表菌株 LM112（=DSM 116216=HAMBI 3782）和 R4（=DSM 116183=HAMBI 3785）分别将这两个种命名为 sp. nov. 和 sp. nov. 。