Rheinische Friedrich-Wilhelms-Universität Bonn, Institut für Ernährungs- und Lebensmittelwissenschaften, Abteilung Lebensmittelmikrobiologie und -hygiene, Meckenheimer Allee 168, 53115 Bonn, Germany.
Rheinische Friedrich-Wilhelms-Universität Bonn, Institut für Tierwissenschaften, Abteilung Physiologie und Hygiene, Katzenburgweg 7-9, 53115 Bonn, Germany.
Int J Syst Evol Microbiol. 2013 Dec;63(Pt 12):4495-4501. doi: 10.1099/ijs.0.050757-0. Epub 2013 Aug 1.
Two groups of Gram-stain positive, aerobic bacterial strains were isolated from raw cow's milk, from a milking machine and from bulk tank milk. Based on their 16S rRNA gene sequences these isolates formed two distinct groups within the genus Corynebacterium. The sequence similarities of the isolates to the type strains of species of the genus Corynebacterium were below 98.4%. The presence of menaquinones MK-8(H2) and MK-9(H2), the predominant fatty acid 18:1 cis 9 and a polar lipid pattern with several phospholipids but without aminolipids was in accord with the characteristics of this genus. The results of DNA-DNA hybridization, biochemical tests and chemotaxonomic properties allowed genotypic and phenotypic differentiation of the strains from all known species of the genus Corynebacterium. Therefore, the isolates were assigned to two novel species of this genus for which the names Corynebacterium frankenforstense sp. nov. (type strain ST18(T) =DSM 45800(T) = CCUG 63371(T)), and Corynebacterium lactis sp. nov. (type strain RW2-5(T) = DSM 45799(T) = CCUG 63372(T)) are proposed, respectively.
两组革兰氏阳性、需氧的细菌菌株是从生牛乳、挤奶机和奶罐奶中分离出来的。根据 16S rRNA 基因序列，这些分离株在棒状杆菌属内形成了两个不同的组。这些分离株与棒状杆菌属种的模式株的序列相似性低于 98.4%。存在menaquinones MK-8(H2)和 MK-9(H2)、主要脂肪酸 18:1 cis 9 和一个极性脂质图谱，其中含有几种磷脂但没有氨基磷脂，这与该属的特征一致。DNA-DNA 杂交、生化试验和化学分类特性的结果允许对这些菌株进行基因型和表型区分，使其与棒状杆菌属的所有已知种都不同。因此，这些分离株被分配到该属的两个新种，分别命名为 Frankenforstense 棒状杆菌（模式株 ST18(T) =DSM 45800(T) = CCUG 63371(T)）和乳棒状杆菌（模式株 RW2-5(T) =DSM 45799(T) =CCUG 63372(T)）。
