Faculty of Environment and Life, Beijing International Science and Technology Cooperation Base of Antivirus Drug, Beijing University of Technology, Beijing 100124, China.
Int J Mol Sci. 2022 Jul 1;23(13):7340. doi: 10.3390/ijms23137340.
This study aims to develop a simple, sensitive, low-cost, environmentally friendly and flexible surface-enhanced Raman scattering (SERS) platform, combined with a portable Raman spectrometer, for the rapid and on-site SERS detection of bacteria. Commercial tobacco packaging paper (TPP) with little background interference was used as a loading medium that effectively adsorbed Au nanoparticles and provided sufficient "hot spots". This Au-tobacco packaging paper (Au-TPP) substrate used as a flexible SERS platform can maximize sample collection by wiping irregular surfaces, and was successfully applied to the on-site and rapid detection of pathogenic bacteria. Raman fingerprints of pathogenic bacteria can be obtained by SERS detection of spiked pork using wipeable Au-TPP, which verifies its value in practical applications. The results collected by SERS were further verified by polymerase chain reaction (PCR) results. It showed several advantages in on-site SERS detection, including accurate discrimination, simple preparation, easy operation, good sensitivity, accuracy and reproducibility. This study indicates that the established flexible SERS platform has good practical applications in pathogenic bacterial identification and other rapid detections.
本研究旨在开发一种简单、灵敏、低成本、环保且灵活的表面增强拉曼散射（SERS）平台，结合便携式拉曼光谱仪，用于快速现场 SERS 检测细菌。商用烟草包装纸（TPP）具有较小的背景干扰，可用作负载介质，有效地吸附金纳米粒子并提供足够的“热点”。这种用作柔性 SERS 平台的 Au-烟草包装纸（Au-TPP）基底可以通过擦拭不规则表面最大限度地收集样本，并成功应用于现场和快速检测致病菌。通过使用可擦拭的 Au-TPP 对涂抹猪肉进行 SERS 检测，可以获得致病菌的 Raman 指纹，这验证了它在实际应用中的价值。SERS 收集的结果进一步通过聚合酶链反应（PCR）结果得到验证。它在现场 SERS 检测中表现出了一些优势，包括准确的区分、简单的制备、易于操作、良好的灵敏度、准确性和重现性。本研究表明，所建立的柔性 SERS 平台在致病菌鉴定和其他快速检测方面具有良好的实际应用前景。
