卵泡抑素过表达的代谢谱分析：一种治疗代谢性疾病的新策略。
Metabolic profiling of follistatin overexpression: a novel therapeutic strategy for metabolic diseases.
作者信息
Singh Rajan, Pervin Shehla, Lee Se-Jin, Kuo Alan, Grijalva Victor, David John, Vergnes Laurent, Reddy Srinivasa T
机构信息
Department of Obstetrics and Gynecology, UCLA School of Medicine, Los Angeles, CA, USA.
Division of Endocrinology and Metabolism, Charles R. Drew University of Medicine and Science, Los Angeles, CA, USA.
出版信息
Diabetes Metab Syndr Obes. 2018 Mar 26;11:65-84. doi: 10.2147/DMSO.S159315. eCollection 2018.
BACKGROUND
Follistatin (Fst) promotes brown adipocyte characteristics in adipose tissues.
METHODS
Abdominal fat volume (CT scan), glucose clearance (GTT test), and metabolomics analysis (mass spectrometry) of adipose tissues from Fst transgenic (Fst-Tg) and wild type (WT) control mice were analyzed. Oxygen consumption (Seahorse Analyzer) and lipidomics (gas chromatography) was analyzed in 3T3-L1 cells.
RESULTS
Fst-Tg mice show significant decrease in abdominal fat content, increased glucose clearance, improved plasma lipid profiles and significant changes in several conventional metabolites compared to the WT mice. Furthermore, overexpression of Fst in 3T3-L1 cells resulted in up regulation of key brown/beige markers and changes in lipidomics profiles.
CONCLUSION
Fst modulates key factors involved in promoting metabolic syndrome and could be used for therapeutic intervention.
背景
卵泡抑素（Fst）可促进脂肪组织中棕色脂肪细胞的特性。
方法
对Fst转基因（Fst-Tg）小鼠和野生型（WT）对照小鼠的腹部脂肪体积（CT扫描）、葡萄糖清除率（葡萄糖耐量试验）以及脂肪组织的代谢组学分析（质谱分析）进行了研究。使用海马分析仪对3T3-L1细胞的耗氧量进行了分析，并采用气相色谱法进行了脂质组学分析。
结果
与WT小鼠相比，Fst-Tg小鼠的腹部脂肪含量显著降低，葡萄糖清除率提高，血浆脂质谱改善，几种传统代谢物也发生了显著变化。此外，在3T3-L1细胞中过表达Fst导致关键棕色/米色标志物上调以及脂质组学谱的改变。
结论
Fst可调节参与促进代谢综合征的关键因素，可用于治疗干预。
Zotero 插件