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MrgprC通过抑制TRPV1通路参与电针镇痛以减轻CFA诱导的热痛觉过敏。

Involvement of MrgprC in Electroacupuncture Analgesia for Attenuating CFA-Induced Thermal Hyperalgesia by Suppressing the TRPV1 Pathway.

作者信息

Liu Ying-Jun, Lin Xiao-Xi, Fang Jian-Qiao, Fang Fang

机构信息

Department of Neurobiology and Acupuncture Research, The Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, China.

出版信息

Evid Based Complement Alternat Med. 2018 Feb 12;2018:9102107. doi: 10.1155/2018/9102107. eCollection 2018.

DOI:10.1155/2018/9102107
PMID:29619074
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5829339/
Abstract

Mas-related G-protein-coupled receptor C (MrgprC) plays an important role in modulating chronic inflammatory pain. Electroacupuncture (EA) has a satisfactory analgesic effect on chronic pain. This study aimed to investigate the involvement of MrgprC and its transient receptor potential vanilloid 1 (TRPV1) pathway in EA analgesia in chronic inflammatory pain. Chronic inflammatory pain was induced by subcutaneously injecting complete Freund's adjuvant (CFA) into the left hind paw. EA (2/100 Hz) stimulation was administered. MrgprC siRNAs were intrathecally administered to inhibit MrgprC expression, and bovine adrenal medulla 8-22 (BAM8-22) was used to activate MrgprC. The mechanical allodynia was decreased by EA significantly since day 3. The piled analgesic effect of EA was partially blocked by 6 intrathecal administrations of MrgprC siRNA. Both EA and BAM8-22 could downregulate the expression of TRPV1 and PKC in both the DRG and the SCDH. Both EA and BAM8-22 could also decrease the TRPV1 translocation and p-TRPV1 level in both the DRG and the SCDH. The effects of EA on PKC, TRPV1 translocation, and p-TRPV1 in both the DRG and the SCDH were reversed by MrgprC siRNA. The results indicated that MrgprC played crucial roles in chronic pain modulation and was involved in EA analgesia partially through the regulation of TRPV1 function at the DRG and SCDH levels.

摘要

Mas相关G蛋白偶联受体C（MrgprC）在调节慢性炎性疼痛中起重要作用。电针（EA）对慢性疼痛具有令人满意的镇痛效果。本研究旨在探讨MrgprC及其瞬时受体电位香草酸亚型1（TRPV1）通路在慢性炎性疼痛电针镇痛中的作用。通过将完全弗氏佐剂（CFA）皮下注射到左后爪诱导慢性炎性疼痛。给予电针（2/100Hz）刺激。鞘内注射MrgprC小干扰RNA（siRNAs）以抑制MrgprC表达，并使用牛肾上腺髓质8-22（BAM8-22）激活MrgprC。自第3天起，电针显著降低了机械性异常性疼痛。鞘内注射6次MrgprC siRNA可部分阻断电针的累积镇痛效果。电针和BAM8-22均可下调背根神经节（DRG）和脊髓背角浅层（SCDH）中TRPV1和蛋白激酶C（PKC）的表达。电针和BAM8-22还可降低DRG和SCDH中TRPV1的转位和磷酸化TRPV1水平。MrgprC siRNA可逆转电针对DRG和SCDH中PKC、TRPV1转位及磷酸化TRPV1的作用。结果表明，MrgprC在慢性疼痛调节中起关键作用，并且部分通过在DRG和SCDH水平调节TRPV1功能参与电针镇痛。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cade/5829339/3b7daaddf62a/ECAM2018-9102107.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cade/5829339/090d73bd9234/ECAM2018-9102107.001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cade/5829339/225ab3408ff5/ECAM2018-9102107.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cade/5829339/9079534551cb/ECAM2018-9102107.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cade/5829339/6356a5879fd4/ECAM2018-9102107.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cade/5829339/3b7daaddf62a/ECAM2018-9102107.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cade/5829339/090d73bd9234/ECAM2018-9102107.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cade/5829339/4bc2466cdd23/ECAM2018-9102107.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cade/5829339/b9bd30a1bb24/ECAM2018-9102107.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cade/5829339/7c2bd31d883c/ECAM2018-9102107.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cade/5829339/fb5840e97e51/ECAM2018-9102107.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cade/5829339/225ab3408ff5/ECAM2018-9102107.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cade/5829339/9079534551cb/ECAM2018-9102107.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cade/5829339/6356a5879fd4/ECAM2018-9102107.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cade/5829339/3b7daaddf62a/ECAM2018-9102107.009.jpg

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