Carvalho Poliana M M, Macêdo Cícero A F, Ribeiro Tiago F, Silva Andressa A, Da Silva Renata E R, de Morais Luís P, Kerntopf Marta R, Menezes Irwin R A, Barbosa Roseli
Physiopharmacology of Excitability Cell Laboratory, Department of Chemical Biology Regional University of Cariri, Campus of Pimenta, 63105-010, Crato, CE, Brazil.
Pharmacology of Natural Products Laboratory, Regional University of Cariri-Campus of Pimenta, 63105-010, Crato, CE, Brazil.
Biotechnol Rep (Amst). 2017 Dec 6;17:31-34. doi: 10.1016/j.btre.2017.12.002. eCollection 2018 Mar.
The (Mill.) N.E. Brown (Verbenaceae) species, has effects sedative, analgesic and spasmolytic properties. This study had as its main objective to evaluate the essential oil of (EOLa) effect and that of its main constituents, citral and limonene, over tracheal smooth muscle from Wistar rats. EOLa, citral and limonene promoted relaxation of tracheal smooth muscle in contractions induced by potassium (60 mM K), presenting an EC of 148 ± 7 μg/mL for the EOLa, 136 ± 7 μg/mL for citral and 581 ± 7 μg/mL for limonene. In contractions induced by Acetylcholine (Ach; 10 μM) the EC for the EOLa and citral were of 731 ± 5 μg/mL and 795 ± 9 μg/mL, respectively. In preparations pre-incubated with 1000 μg/mL of the EOLa and citral, both agents were found to block the influx of BaCl by VOCCs. This study demonstrated that the EOLa and its main component citral present antispasmodic effect over tracheal smooth muscle of rats.
（唇形科）N.E. 布朗的该物种具有镇静、镇痛和解痉特性。本研究的主要目的是评估（某种植物名称未完整给出，推测为相关植物）挥发油（EOLa）及其主要成分柠檬醛和柠檬烯对Wistar大鼠气管平滑肌的作用。EOLa、柠檬醛和柠檬烯可促进由钾（60 mM K）诱导收缩的气管平滑肌舒张，EOLa的半数有效浓度（EC）为148±7 μg/mL，柠檬醛为136±7 μg/mL，柠檬烯为581±7 μg/mL。在由乙酰胆碱（Ach；10 μM）诱导的收缩中，EOLa和柠檬醛的EC分别为731±5 μg/mL和795±9 μg/mL。在预先用1000 μg/mL的EOLa和柠檬醛孵育的制剂中，发现这两种物质均可通过电压门控钙通道（VOCCs）阻断BaCl的内流。本研究表明，EOLa及其主要成分柠檬醛对大鼠气管平滑肌具有解痉作用。
