Biological Chemistry Department, Postgraduate Program in Molecular Bioprospecting, Regional University of Cariri, Pimenta Campus, 63105-010, Crato, CE, Brazil.
Biological Chemistry Department, Postgraduate Program in Biological Chemistry, Regional University of Cariri, Pimenta Campus, 63105-010, Crato, CE, Brazil.
Chem Biol Interact. 2019 Jan 5;297:155-159. doi: 10.1016/j.cbi.2018.11.006. Epub 2018 Nov 14.
The species Lippia alba (Mill.) N. E. Brown belongs to the Verbenaceae family. It is abundant and grows spontaneously throughout the Brazilian territory. Popularly known as "erva-cidreira", it is widely used because of its sedative, carminative and analgesic properties. The objective of this study was to investigate the mechanism of action of the L. alba essential oil (EOLa) and its major constituents citral and limonene, on isolated rat uterus muscle. To evaluate the EOLa, citral and limonene effect, cumulative concentrations curves for EOLa and citral (1-600 μg/mL) and for limonene (1-1200 μg/mL) were constructed from contractions of rat uterine strips under a 1 g tension. EOLa, citral and limonene dose-dependently relaxed myometrial preparations pre-contracted with 60 mM KCl, 10-2 IU/mL oxytocin, serotonin (10 μM), or ACh (10 μM). The results demonstrate that the EOLa, citral and limonene cause relaxation of the uterine smooth muscle. These results suggest that the relaxation induced by EOLa, citral and limonene is caused by inhibition of L-type VOCC, inhibiting the Ca current through these channels, although other mechanisms of action are likely to contributing to relaxant activity. There was no involvement of K channels (BKca, K, K) or cyclooxygenase on the relaxation promoted by EOLa. Then studies of the tocolytic effects of EOLa, citral and limonene may yield new insights into their therapeutic use.
利帕氏白花（Mill.）N. E. 布朗物种属于马鞭草科。它在巴西领土上丰富且自然生长。俗称“酸橙草”，因其具有镇静、驱风、止痛的特性而被广泛使用。本研究旨在探讨利帕氏白花精油（EOLa）及其主要成分柠檬醛和柠檬烯对离体大鼠子宫肌肉的作用机制。为了评估 EOLa、柠檬醛和柠檬烯的作用，构建了 EOLa 和柠檬醛（1-600μg/mL）以及柠檬烯（1-1200μg/mL）对大鼠子宫条收缩的累积浓度曲线，张力为 1g。EOLa、柠檬醛和柠檬烯剂量依赖性地松弛了用 60mM KCl、10-2IU/mL 催产素、血清素（10μM）或 ACh（10μM）预收缩的子宫肌层制剂。结果表明，EOLa、柠檬醛和柠檬烯导致子宫平滑肌松弛。这些结果表明，EOLa、柠檬醛和柠檬烯引起的松弛是通过抑制 L 型 VOCC 引起的，通过这些通道抑制钙电流，尽管其他作用机制可能有助于松弛活性。EOLa 促进的松弛不涉及钾通道（BKca、K、K）或环氧化酶。然后，对 EOLa、柠檬醛和柠檬烯的保胎作用的研究可能会为其治疗用途提供新的见解。
