Sucipto Teguh Hari, Churrotin Siti, Setyawati Harsasi, Martak Fahimah, Mulyatno Kris Cahyo, Amarullah Ilham Harlan, Kotaki Tomohiro, Kameoka Masanori, Yotopranoto Subagyo, Soegijanto And Soegeng
Dengue Study Group, Institute of Tropical Disease, Airlangga University, Kampus C Jl. Mulyorejo, Surabaya, East Java, Indonesia, 60115.
Department of Chemistry, Faculty of Science and Technology, Airlangga University, Kampus C Jl. Mulyorejo, Surabaya, East Java, Indonesia, 60115.
Afr J Infect Dis. 2018 Mar 7;12(1 Suppl):116-119. doi: 10.2101/Ajid.12v1S.17. eCollection 2018.
Dengue is a kind of infectious disease that was distributed in the tropical and sub-tropical areas. To date, there is no clinically approved dengue vaccine or antiviral for humans, even though there have been great efforts towards this end. Therefore, finding the effective compound against dengue virus (DENV) replication is very important. Among the complex compounds, copper(II)-imidazole derivatives are of interest because of their biological and medicinal benefits.
In the present study, antiviral activity of [Cu(2,4,5-triphenylimidazole)], was evaluated against different stages of dengue virus type 2 (DENV-2) replication in Vero cell using focus forming unit reduction assay and quantitative ELISA.
[Cu(2,4,5-triphenylimidazole)] inhibited DENV-2 replication in Vero cells with IC = 2.3 μg/ml and SI= 19.42 when cells were treated 2 days after virus infection, whereas its CC for cytotoxicity to Vero cells was 44.174 μg/ml.
The compound has high anti-DENV2 activity, less toxicity, and a high possibility to be considered a drug candidate.
登革热是一种分布于热带和亚热带地区的传染病。迄今为止，尽管为此付出了巨大努力，但尚无临床批准的用于人类的登革热疫苗或抗病毒药物。因此，寻找针对登革热病毒（DENV）复制的有效化合物非常重要。在复杂化合物中，铜（II）-咪唑衍生物因其生物学和医学益处而备受关注。
在本研究中，使用蚀斑形成单位减少试验和定量酶联免疫吸附测定法，评估了[Cu(2,4,5-三苯基咪唑)]对Vero细胞中登革热病毒2型（DENV-2）复制不同阶段的抗病毒活性。
当在病毒感染2天后处理细胞时，[Cu(2,4,5-三苯基咪唑)]抑制Vero细胞中DENV-2复制，IC = 2.3 μg/ml，SI = 19.42，而其对Vero细胞的细胞毒性CC为44.174 μg/ml。
该化合物具有高抗DENV2活性、低毒性，很有可能被视为候选药物。
