Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran; College of Veterinary Medicine, AlQadisiyiah University, Diwanyiah, Iraq.
Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
Cytokine. 2019 Jan;113:265-271. doi: 10.1016/j.cyto.2018.07.016. Epub 2018 Jul 30.
To determine the prognostic value of intrafollicular concentrations of some cytokines from women undergoing ovarian stimulation in the outcome of intracytoplasmic sperm injection/embryo transfer (ICSI/ET) cycles.
A total of 80 patients were included in this study following ovarian stimulation and ICSI. Follicular fluids (FF) were collected at the day of oocyte retrieval. Ten cytokines including: tumor necrosis factor- alpha (TNF-α), interleukin (IL)-1β, IL-2, IL-4, IL-5, IL-6, CXCL8/IL-8, IL-10, granulocyte-macrophage colony stimulating factor (GM-CSF), and interferon gamma (IFN-γ) were measured using magnetic multiplex immunoassays.
Only the concentration of IL-5, IL-4, and GM-CSF in FF were significantly different (p < 0.05) between ICSI cycles that resulted in pregnancy and those that failed. Elevated FF IL-5 levels were associated with poor oocyte quality, which decreases the chance of both biochemical and clinical pregnancy. Higher FF GM-CSF associated with decrease of mature oocytes, while higher FF IL-4 concentrations were linked to good ICSI outcome through increased fertilization rate.
The elevated intrafollicular concentrations of IL-5 seem to be a negative predictor to the pregnancy outcome in ICSI cycles.
确定接受卵巢刺激的女性卵泡内的某些细胞因子浓度对胞浆内精子注射/胚胎移植（ICSI/ET）周期结局的预测价值。
本研究共纳入 80 例接受卵巢刺激和 ICSI 的患者。在取卵日采集卵泡液（FF）。使用磁性多重免疫分析检测 10 种细胞因子，包括肿瘤坏死因子-α（TNF-α）、白细胞介素（IL）-1β、IL-2、IL-4、IL-5、IL-6、CXCL8/IL-8、IL-10、粒细胞-巨噬细胞集落刺激因子（GM-CSF）和干扰素γ（IFN-γ）。
只有 FF 中 IL-5、IL-4 和 GM-CSF 的浓度在导致妊娠和失败的 ICSI 周期之间存在显著差异（p<0.05）。FF 中升高的 IL-5 水平与卵子质量差有关，这降低了生化和临床妊娠的机会。较高的 FF GM-CSF 与成熟卵母细胞减少有关，而较高的 FF IL-4 浓度通过增加受精率与良好的 ICSI 结局相关。
卵泡内升高的 IL-5 浓度似乎是 ICSI 周期妊娠结局的负预测因子。
