粗糙脉孢菌中一种新型的蛋白质磷酸化刺激剂。

A novel stimulator of protein phosphorylation in Neurospora crassa.

作者信息

Ulloa R M, Glikin G C, Téllez-Iñón M T, Torres H N, Judewicz N D

机构信息

Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI) CONICET, Buenos Aires, Argentina.

出版信息

Mol Cell Biochem. 1987 Sep;77(1):11-7. doi: 10.1007/BF00230146.

DOI:10.1007/BF00230146

PMID:2961977

Abstract

An endogenous thermostable activator of Protein kinase III (PKIII) was purified from 100,000 X g supernatants of Neurospora crassa mycelial extracts. This 38,000 dalton polypeptide, clearly separable from calmodulin on P-60 gel filtration, specifically stimulated N. crassa PKIII activity on casein or phosvitin "in vitro" phosphorylation. The factor was only present in the initial growth phase of the fungus. The mechanism of PKIII activation and its possible regulatory role are discussed.

摘要

从粗糙脉孢菌菌丝体提取物的100,000×g上清液中纯化出一种蛋白激酶III（PKIII）的内源性热稳定激活剂。这种38,000道尔顿的多肽在P-60凝胶过滤中可与钙调蛋白清晰分离，在“体外”酪蛋白或卵黄高磷蛋白磷酸化过程中能特异性刺激粗糙脉孢菌PKIII的活性。该因子仅存在于真菌的初始生长阶段。文中讨论了PKIII激活的机制及其可能的调节作用。