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马铃薯(Solanum tuberosum L.)微型块茎形成不同阶段的蛋白激酶活性

Protein kinase activity in different stages of potato (Solanum tuberosum L.) microtuberization.

作者信息

Ulloa R M, Mac Intosh G C, Melchiorre M, Mentaberry A N, Dallari P, Moriconi D N, Téllez-Iñón M T

机构信息

Genética y Biología Molecular (INGEBI-CONICET), Instituto Investigaciones en Ingeniería, Via. Obligado 2490, (l428), Buenos Aires, Argentina.

Laboratorio de Biotecnología Vegetal Fac. Ciencias Agropecuarias, Univ. Nac. de Córdoba, CC 509, (5000), Córdoba, Argentina.

出版信息

Plant Cell Rep. 1997 Mar;16(6):426-429. doi: 10.1007/BF01146787.

Abstract

In vitro culture was used to study morphogenetic aspects of the tuberization process under controlled conditions in potato (Solanum tuberosum L.) plants. This paper accurately defines four stages of tuber development and their correlation to external morphological characteristics and histological structures. Protein kinase activity, assayed in each stage using Historic HAS as substrate, was differentially expressed during the tuberization process. Phosphorylation was maximum in the first stages of tuber formation. The incorporation of [PO ] to endogenous peptides containing serine/threonine amino acidic residues followed the same pattern that the protein kinase activity did.

摘要

利用体外培养技术,在可控条件下研究马铃薯(Solanum tuberosum L.)植株块茎形成过程的形态发生方面。本文准确界定了块茎发育的四个阶段及其与外部形态特征和组织结构的相关性。在每个阶段以组蛋白HAS为底物测定的蛋白激酶活性,在块茎形成过程中呈差异表达。磷酸化在块茎形成的最初阶段最高。[PO]掺入含丝氨酸/苏氨酸氨基酸残基的内源性肽的模式与蛋白激酶活性相同。

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