School of Food and Biological Engineering, Jiangsu University, Zhenjiang, China.
School of Agricultural Engineering, Jiangsu University, Zhenjiang, China.
Arch Microbiol. 2021 Dec 31;204(1):106. doi: 10.1007/s00203-021-02638-3.
An alkaliphilic and aerobic bacterium, designated as strain JB21, was isolated from a soda alkali-saline soil sample in Heilongjiang, Northeast China. Strain JB21 is a Gram-stain-negative, rod-shaped, non-motile and amylase-positive bacterium. Growth occurred at 15-45 °C (optimum, 35-37 °C), in the presence of 0-15.0% (w/v) NaCl (optimum, 1.0%) and at pH 6.5-10.5 (optimum, pH 8.5-9.5). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain JB21 was most closely related to type strains of the genus Alcanivorax, with the highest sequence similarity to Alcanivorax indicus SW127 (96.3%), and shared 95.4-93.1% sequence identity with other valid type strains of this genus. The major cellular fatty acids identified were C and summed feature 8 (Cω6c and/or Cω7c). The polar lipids comprised phosphatidylethanolamine, phosphatidylglycerol and one unidentified phospholipid. The genomic G + C content of strain JB21 was 61.3 mol%. The digital DNA-DNA hybridization (dDDH) estimation and average nucleotide identity (ANI) between strain JB21 and type strains of the genus Alcanivorax were 18.3-23.2% and 69.2-79.0%, respectively. On the basis of its phenotypic and phylogenetic characteristics, we suggest the creation of a new species within the Alcanivorax genus, named Alcanivorax limicola sp. nov., type strain JB21 (= CGMCC 1.16632 = JCM 33717).
从中国东北黑龙江的苏打盐碱土壤样本中分离到一株嗜碱好氧细菌，命名为 JB21 菌株。JB21 菌株是革兰氏阴性、杆状、非运动和淀粉阳性细菌。在 15-45°C（最佳，35-37°C）、0-15.0%（w/v）NaCl（最佳，1.0%）和 pH 6.5-10.5（最佳，pH 8.5-9.5）的条件下生长。基于 16S rRNA 基因序列的系统发育分析表明，JB21 菌株与 Alcanivorax 属的模式菌株最为密切相关，与 Alcanivorax indicus SW127 的序列相似度最高（96.3%），与该属其他有效模式菌株的序列相似度为 95.4-93.1%。鉴定的主要细胞脂肪酸为 C 和总和特征 8（Cω6c 和/或 Cω7c）。极性脂包括磷脂酰乙醇胺、磷脂酰甘油和一种未鉴定的磷脂。JB21 菌株的基因组 G+C 含量为 61.3 mol%。JB21 菌株与 Alcanivorax 属模式菌株的数字 DNA-DNA 杂交（dDDH）估计值和平均核苷酸同一性（ANI）分别为 18.3-23.2%和 69.2-79.0%。基于其表型和系统发育特征，我们建议在 Alcanivorax 属内创建一个新种，命名为嗜盐 Limicola 亚种 Alcanivorax limicola sp. nov.，模式菌株 JB21（=CGMCC 1.16632=JCM 33717）。
