Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, Paraná, 81.531-980, Brazil.
Laboratório Glucoconjugados Bioativos, Departamento de Bioquímica, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte 59.078-970, Brazil.
Food Chem. 2018 Aug 15;257:143-149. doi: 10.1016/j.foodchem.2018.02.122. Epub 2018 Feb 24.
Three polysaccharide fractions were isolated from blackberry wine. The crude extract BWPs was obtained with ethanol precipitation and freeze-thawing process, it was then submitted to Fehling treatment, giving soluble BWPFs and insoluble BWPFp fractions. These fractions were characterized by Gas Chromatography-Mass Spectrometry (GC-MS) and Nuclear Magnetic Resonance (NMR). Major polysaccharides were identified for each fraction: mannan, type II arabinogalactan and type I rhamnogalacturonan for BWPs, a mannan formed by a major chain of α-Manp(1 → 6)-linked units, O-2 substituted with α-d-Manp(1 → 2)-linked side chains for BWPFp and a AG II formed by a major chain of β-d-Galp(1 → 3)-linked, substituted at O-6 by side chains of the β-d-Galp(1 → 6)-linked, which then are substituted at O-3 by non-reducing units of α-l-Araf and a RG I, formed by [→4)-α-d-GalpA-(1 → 2)-α-l-Rhap-(1→] for BWPFs. Anti-inflammatory effects of polysaccharide fractions were evaluated in RAW 264.7 cells. Fractions markedly reduced nitric oxide (NO) and pro-inflammatory cytokine production (TNF-α and IL-1β) in LPS-treated cells.
从黑莓酒中分离出三种多糖级分。粗提物 BWPs 通过乙醇沉淀和冻融过程获得，然后进行菲林处理，得到可溶的 BWPFs 和不溶的 BWPFp 级分。这些级分通过气相色谱-质谱（GC-MS）和核磁共振（NMR）进行了表征。确定了每个级分的主要多糖：BWPs 中的甘露聚糖、Ⅱ型阿拉伯半乳聚糖和Ⅰ型鼠李半乳糖醛酸聚糖，BWPFp 中的甘露聚糖由主要的α-Manp(1 → 6)-连接单元组成，O-2 被α-d-Manp(1 → 2)-连接的侧链取代，BWPFs 中的 AG II 由β-d-Galp(1 → 3)-连接的主要链组成，在 O-6 处被β-d-Galp(1 → 6)-连接的侧链取代，然后在 O-3 处被非还原的α-l-Araf 和 RG I 取代，RG I 由[→4)-α-d-GalpA-(1 → 2)-α-l-Rhap-(1→组成。评估了多糖级分对 RAW 264.7 细胞的抗炎作用。各部分在 LPS 处理的细胞中显著降低了一氧化氮（NO）和促炎细胞因子（TNF-α和 IL-1β）的产生。
