Holán V
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Prague.
Transplantation. 1987 Dec;44(6):809-12.
Inoculation of newborn mice with spleen and bone marrow cells from newborn (less than 24-hr-old) rat donors, as compared with cells from adult donors, resulted in induction of profound hyporeactivity or even long-lasting tolerance of skin xenografts. This tolerance was specific since skin allografts transplanted to tolerized mice simultaneously with specific rat grafts were rejected in normal fashion. Analysis of the reactivity in vitro in mixed lymphocyte culture and cytotoxicity assays revealed that cells from tolerant animals were specifically nonreactive to the tolerated xenogeneic antigens. Administration of active supernatants from cultures of concanavalin A-stimulated spleen cells from adult rats to tolerized mice within the first 5 days after birth impaired tolerance. The results show that the approach used is the first successful model of neonatal induction of tolerance to skin xenografts in mammals and emphasize the role of immunoregulatory factors in this process.
用新生（小于24小时龄）大鼠供体的脾脏和骨髓细胞接种新生小鼠，与用成年供体的细胞相比，可诱导皮肤异种移植物产生深度低反应性甚至长期耐受性。这种耐受性具有特异性，因为与特定大鼠移植物同时移植到耐受小鼠的皮肤同种异体移植物以正常方式被排斥。在混合淋巴细胞培养和细胞毒性试验中对体外反应性的分析表明，来自耐受动物的细胞对耐受的异种抗原具有特异性无反应性。在出生后的头5天内，将成年大鼠伴刀豆球蛋白A刺激的脾细胞培养物的活性上清液给予耐受小鼠，会损害耐受性。结果表明，所采用的方法是哺乳动物中新生期诱导对皮肤异种移植物耐受性的首个成功模型，并强调了免疫调节因子在此过程中的作用。
