Van Leeuwen P A, Drukker J, Van der Kleyn N M, Van den Boogaard A E, Soeters P B
Dept. of Surgery, University of Limburg, Maastricht, The Netherlands.
Acta Morphol Neerl Scand. 1986;24(3):223-34.
The morphology of the intestinal wall and the activity of certain mucosal enzyme systems in the course of neomycin treatment were evaluated. Conventional and, to study the role of the bacterial flora, germ-free rats received 500 mg neomycin daily by stomach tube. Rats were sacrificed after seven days and small intestine (proximal and distal part) together with segments of the colon were removed and prepared for histochemistry. The colon and proximal small intestine of untreated conventional and germ-free animals did not show appreciable differences in staining activity after treatment with neomycin. Neomycin diminished both in normal and germ-free rats the activity of NAD tetrazolium reductase, succinate dehydrogenase, esterase, alkaline phosphatase and acid phosphatase in the distal small intestine. The findings of this study indicate that explanations for the beneficial effects of neomycin on hyperammonemia in liver disease should not only include the bactericidal action of neomycin but also its influence on absorption and metabolic functions of the mucosal cells.
评估了新霉素治疗过程中肠壁的形态以及某些黏膜酶系统的活性。为了研究细菌菌群的作用，常规大鼠和无菌大鼠通过胃管每日接受500毫克新霉素。七天后处死大鼠，取出小肠（近端和远端部分）以及结肠段并进行组织化学制备。未经治疗的常规和无菌动物的结肠和近端小肠在用新霉素治疗后染色活性没有明显差异。新霉素使正常大鼠和无菌大鼠远端小肠中的NAD四氮唑还原酶、琥珀酸脱氢酶、酯酶、碱性磷酸酶和酸性磷酸酶的活性均降低。本研究结果表明，对新霉素治疗肝病时高氨血症有益作用的解释不仅应包括新霉素的杀菌作用，还应包括其对黏膜细胞吸收和代谢功能的影响。
