Abrass C K, Cohen A H
Division of Nephrology, Veterans Administration Medical Center, Seattle, WA 98108.
Am J Pathol. 1988 Jan;130(1):103-11.
To investigate the role of antibody to heparan sulfate (HS) in the development of glomerular injury, male Lewis rats were immunized with HS and compared with unimmunized controls. In HS-immunized rats circulating antibodies that bound to renal basement membranes, an increase in serum creatinine (0.8 mg/dl versus 0.6 in controls P less than 0.01), and a 40% decline in creatinine clearance developed. In no animal did abnormal proteinuria develop. By histologic examination there was glomerular and interstitial capillary engorgement with erythrocytes, modest infiltration by polymorphonuclear leukocytes, and no proliferation of intrinsic glomerular cells. Immunofluorescence microscopy demonstrated deposits of rat IgG along the glomerular basement membrane. Bowman's capsule, and peritubular capillaries. Electron-microscopic examination revealed capillary engorgement with erythrocytes that appeared adherent to each other and contained entrapped areas of rarefied material. These observations demonstrate that binding of antibody to HS in the glomerulus induces a mild inflammatory reaction and a reduction in glomerular filtration rate, but no abnormal proteinuria.
为研究硫酸乙酰肝素（HS）抗体在肾小球损伤发展中的作用，将雄性Lewis大鼠用HS免疫，并与未免疫的对照大鼠进行比较。在HS免疫的大鼠中，出现了与肾基底膜结合的循环抗体，血清肌酐升高（0.8mg/dl，对照组为0.6mg/dl，P<0.01），肌酐清除率下降40%。没有一只动物出现异常蛋白尿。组织学检查显示肾小球和间质毛细血管红细胞充盈，多形核白细胞轻度浸润，肾小球固有细胞无增殖。免疫荧光显微镜检查显示大鼠IgG沿肾小球基底膜、鲍曼囊和肾小管周围毛细血管沉积。电子显微镜检查显示毛细血管红细胞充盈，红细胞相互粘连，含有稀疏物质的包埋区域。这些观察结果表明肾小球中抗体与HS的结合可诱导轻度炎症反应和肾小球滤过率降低，但无异常蛋白尿。
