Stow J L, Sawada H, Farquhar M G
Proc Natl Acad Sci U S A. 1985 May;82(10):3296-300. doi: 10.1073/pnas.82.10.3296.
A polyclonal antibody was raised against a proteoglycan fraction purified from extracts of isolated rat renal glomeruli. The antibody was characterized by column chromatography and by NaDodSO4/PAGE analysis of immunoprecipitates and was shown to recognize specifically the core protein of a population of heparan sulfate proteoglycans (Mr 130,000) found in the glomerular basement membrane and in other renal basement membranes. The antibody was used to localize the core proteins of this population of heparan sulfate proteoglycans (HSPG) in the glomerulus by immunoelectron microscopic techniques. These HSPG were found to be concentrated in the lamina rarae interna and externa of the glomerular basement membrane (GBM) and precursor forms were detected in the endoplasmic reticulum and Golgi cisternae of glomerular visceral epithelial cells (podocytes).
制备了一种针对从大鼠分离的肾小球提取物中纯化的蛋白聚糖组分的多克隆抗体。通过柱色谱以及免疫沉淀物的十二烷基硫酸钠/聚丙烯酰胺凝胶电泳（NaDodSO4/PAGE）分析对该抗体进行了表征，结果表明它能特异性识别在肾小球基底膜和其他肾基底膜中发现的一组硫酸乙酰肝素蛋白聚糖（分子量130,000）的核心蛋白。利用该抗体通过免疫电子显微镜技术在肾小球中定位这组硫酸乙酰肝素蛋白聚糖（HSPG）的核心蛋白。发现这些HSPG集中在肾小球基底膜（GBM）的内、外疏松层，并且在肾小球脏层上皮细胞（足细胞）的内质网和高尔基池中检测到前体形式。
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